Talk:2016 Lab 2

From CellBiology


<mediaplayer width='500' height='300'></mediaplayer>

Sci Adv. 2015 Nov 13;1(10):e1500947. doi: 10.1126/sciadv.1500947. eCollection 2015. Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth. Ishitsuka Y1, Savage N2, Li Y1, Bergs A3, Grün N3, Kohler D1, Donnelly R2, Nienhaus GU4, Fischer R3, Takeshita N5. Author information Abstract Polar (directional) cell growth, a key cellular mechanism shared among a wide range of species, relies on targeted insertion of new material at specific locations of the plasma membrane. How these cell polarity sites are stably maintained during massive membrane insertion has remained elusive. Conventional live-cell optical microscopy fails to visualize polarity site formation in the crowded cell membrane environment because of its limited resolution. We have used advanced live-cell imaging techniques to directly observe the localization, assembly, and disassembly processes of cell polarity sites with high spatiotemporal resolution in a rapidly growing filamentous fungus, Aspergillus nidulans. We show that the membrane-associated polarity site marker TeaR is transported on microtubules along with secretory vesicles and forms a protein cluster at that point of the apical membrane where the plus end of the microtubule touches. There, a small patch of membrane is added through exocytosis, and the TeaR cluster gets quickly dispersed over the membrane. There is an incessant disassembly and reassembly of polarity sites at the growth zone, and each new polarity site locus is slightly offset from preceding ones. On the basis of our imaging results and computational modeling, we propose a transient polarity model that explains how cell polarity is stably maintained during highly active directional growth. KEYWORDS: Aspergillus; Computational Modeling; PALM; exocytosis; filamentous fungi; microtubule; polarity maintenance; super-resolution microscopy PMID 26665168

Lab 2 Individual Assessment

Identify a research article (not review) on super resolution microscopy and prepare a brief (1-2 paragraph) summary of the paper. Do not just use the paper abstract, the paper must be fully available online. Add the referenced summary to your page.