Talk:2011 Group 3 Project

From CellBiology

Post Peer Assessment Discussion

Hey guys in response to the peers i moved around my pictures, gave them text under them, and added a lot of new information and some photos to the structure section. the only issue we have left is to clean up our reference list since it mostly looks like a bunch of pubmed IDs. i guess we will ask mark what to do with this.--Michael Orenstein 21:48, 25 May 2011 (EST)

Week 7

  • This is the week before the mid-session break.
  • In the lab this week we will have an opportunity to discuss any issues which are slowing progress on your project.
  • The Thursday of the week beginning 02 May will be when all projects will be open to Peer Assessment.
  • What you have on your page by Thursday of that week will be the content that others in the class will comment upon.

Week 6

  1. I see many groups now have subsection titles for their projects.
  2. Here are some searches: Pubmed search all databases junction | PLoS junction | JCB junction You can now simply put your own search term into each top window.
  3. Now's the time to get your images, movies, media etc uploaded. Biomed central | JCB | JCB Archive | PLoS. Once uploaded you can make a gallery on either your project or discussion page using <gallery>File:name here</gallery> tags with your image files listed between the tags. When you upload project images, add this text as it appears replacing # with your own Group number to the summary information [[Category:2011Project#]].
  4. Shown below are the criteria that will be used to assess your final project.

Group Assessment Criteria

  • The key points relating to the topic that your group was allocated are clearly described.
  • The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  • Content is correctly cited and referenced.
  • The wiki has an element of teaching at a peer level using the student’s own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  • Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  • Relates the topics and content of the Wiki entry to learning aims of cell biology.
  • Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer’s wiki.
  • Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  • The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  • Develops and edits the wiki entries in accordance with this sites wiki guidelines.


By Week 5

Each Group member has added to the discussion page:

  1. A Review Article
  2. A Historic Research Article
  3. A Current Research Article

No two students should add the same paper and there should be a link to the original article.

Peer Review

Group 3:

  • Very good introduction, giving a good overview of all junctions and then specifically what a tight junction, very informative for the reader. But maybe write a bit more about tight junctions than the other junctions to emphasize what your page will be about.
  • Good history section, is there anything beyond 2005?
  • Maybe in structure could bold some key words like the main proteins occludin and claudin to emphasize this to the reader like you have in molecular components. Maybe make molecular components a subheading under structure as that what your essentially talking about. Use of dot points in this section is very useful.
  • Could split the information in function and "Classification of Epithelia Using Tight Junctions" into dot points to make is easier to read, or even just smaller paragraphs as the big blocks of information is more difficult to take in.
  • Diseases is very well researched and detailed but how are these diseases caused?
  • Glossary needs to be expanded, every scientific term in the page should be explained here.
  • Overall the page is very well researched and presented with a good balance of pictures.


Group 3 Tight Junctions:

I like how you manage to use photos in each section, which makes the reader keen to look through the content. History is the

appropriate length of information. As for the Structure: The photo on the left could be resized to smaller. The use of dot points

is a great way and it makes it easier to read.

Maybe for the classification section, It would be alot easier if you make it as a table.

Finally, the Table of Diseases is remarkable. Shows good description of all the diseases mentioned.

I think for the Glossary, Having such a deep understanding of the Topic, It be expected to be more than 7 words. References are a

minor issue, it can be fixed later on.

Overall, The page shows a deep research and cooperation between the group members.


GROUP 3: Tight Junctions


Your project is put together very well in terms of its structure and layout, making it easy to keep up with the expanse of information you have provided. The detail and depth you've gone into is very impressive especially in the introduction and history sections. The use of tables is especially effective in the disease sections because I feel it presents the content in a clear and concise way making it easier for the reader to understand and make comparisons. The glossary also must be completed. Also in the function section I feel the image to text ratio isn’t right. There were some small spelling and grammatical mistake but that should be fine when you go over it before final submission Otherwise, the page is very well thought out and I definitely felt like I gathered a thorough overview from it.


Group 3

Good concise introduction.

History well balanced with the addition of pictures.

Molecular components is detailed, but readable.

Spelling error in one of your subheadings in function.

Content in classification of epithelia is good, but it gets very tiring reading this so try breaking this up.

Very extensive table of diseases but how do tight junctions actually cause these??

Might also be worthwhile adding to your glossary, but overall good project.


Group3 – Tight junctions

  • The introduction was clear and very informative .
  • History section was too detailed – should break in down into dot points and with lesser text
  • the molecular sections are beautifully represented however, there are some sections with missing photos
  • Classification of epithelia needs to be cut down into points or bolded text
  • The diesease section was very well presented into table may be try summarising the idea

Overall the project looked awesome :)


Group 3

The introduction is simple and clear. However, the picture “Schematic of the various cell-cell junctions” is lacking copyright and reference information. The history is clear and concise but seems to be lacking any important discoveries from the 1950s and 1970s. Perhaps there were none made during this time period but that gap jumped out at me. In the Structure section, there seems to be a lack of referencing at the beginning of the section. The picture titled “Schematic model of the basic structural transmembrane components of tight junctions” is lacking copyright and referencing information. The group may mean that this picture was made by them but I can’t tell so perhaps they should make it clearer if that is the case. I found the use of bullet points to display the roles of occludins, claudins and other proteins very helpful and clear. This makes for an easy read for the reader. The structure section was extremely informative and easy to read. The Barrier Function section is missing some content. It says “If the tight junction were not present then solutes from could…” Solutes from what? For the “Transcellular and Paracellular Transport section, I would say to maybe put the first use of those 2 words in bold so that the reader can easily find their definition. The picture with the legend “Circuit Model for the Measure of TER” is missing copyright information. Under the section “Classification of Epithelia Using Tight Junctions”, when the paragraph starts to talk about TER and circuit models I find that it becomes a bit confusing. There are a couple of run on sentences there that, if fixed, would make it way easier to comprehend the information. I liked how the “Disease” section provides a brief introduction into how tight junctions can malfunction followed by a table which provides a brief summary on many of the diseases affiliated with abnormal tight junctions.

Group 3:

  • Good introduction and history
  • Great pictures and tables
  • i know we arent supposed to be commenting on structure but molecular components should be a subheading of structure (add an extra = to each side of heading)
  • glossary is incomplete
  • Disease pictures made me want to read more, loved them :)
  • Clearly a well researched disease table, heaps of references and links!
  • Overall great job guys!


3)

• The intro and history are both good as they provide a broad beginning to set up the page.

• Pictures are well positioned.

• The content in the structure section is fine but the texts location could be changed so it all fits together better.

• Molecular components provides in depth knowledge but was difficult to get through and learn, a table could make this easier to digest.

• Functions was well structures with headings and the diseases table was spot on.

• Future research is needed perhaps on research into the cures or treatments for some diseases.



Group 3: Tight junction

•Introduction needs to focus on your own junction more

•History is well done but anything beyond 2005?

•Glossary needs work –to short

•The disease section is very good but a long read could there be a way to integrate more pictures to help visualise the diseases?

•Similarly for function great information but picture integration will help readers understand better- it is a lot of text.

•Structure is well researched

•Remember to fix all referencing and check spelling before the due date


Group 3 Peer Review

Are there any recent discoveries regarding tight junctions after 2005? History could be a little bit more up to date.

The introduction provides a broad overview of junctions and then explains what tight junction is. A good introduction to start off with!

Just an advice on introduction is to break off words a bit so that it’s not a chunk of paragraph and bore the reader to read through.

The disease section should have more pictures since it’ll attract the reader to read more but the organisation of the information into tables makes it nice and simple to read.

Glossary is too short compared to a lot of scientific vocabulary that is used.

Overall, it is a very well researched and documented page about tight junctions!


Group 3-Tight Junction

  • The page content is very well structured and descriptions are very logical and accessible. Good use of pictures.
  • Disease – the introduction to this section is well written. However, for the table, specific mechanisms involving tight junction in the disease process relating to a few diseases would be better than a rather long list of diseases.
  • Addition of current and future researches may be necessary.
  • Glossary can be expanded by adding terms such as anastomosing etc


Group 3

This page is really informative and I found the intro with the accompanying picture immediately engaging. This page is complimented by several really helpful diagrams that allow the reader to easily picture tight junctions between cells, and how they perform their functions. Glossary should be a bit bigger,there are quite a few technical terms. The section molecular components is well written as there is not an overload of text. The other sections do contain more text which is overwhelming at first impression, but reading through seems reasonable given the content. Perhaps one more example under "Examples of Tight Junctions of the Body" would be beneficial, the section is great, but two examples perhaps doesn't show how big a role they play in the body. Overall, the page is a great resource for anyone looking into tight junctions


Tight junction pro 3

  • Good amount of information and even distribution with each sub heading catered for with photos however some photo placement rearrangement might be good.
  • Table is outstanding and a very good example of how wikipedia can be used as a teaching tool
  • History is clear, other sub headings are wordy
  • A few typos, and glossary could be more extensive
  • The structure section is easy to understand
  • Barrier function needs editing but this can easily be done with what is there and should be no problem
  • There is evidence of group communication
  • Some referencing edits for pictures need to be updated
  • long paragraphs could be broken down a little I am not sure the best way of doing this, as I have had the same problem in formatting my own work, maybe bullet points?



GROUP 3 REVIEW

  • Introduction: Check spelling and break up text to make it easier to read. Images also need copyright info and a link to the website they came from.
  • History: Great
  • Structure: Images also need copyright info and a link to the website they came from. This section really should include Molecular structure components....
  • Molecular Structure Components: This should be under structure and not its own heading I think, by making it bold it will do the trick. Similar to the Tight Junction Function subheading, that really works well.
  • Tight Junction Functions: Great content, Maybe move the pictures around a little so it's not just text one one side, images on the other.
  • Examples of TJ in the Body: Great idea with this section and well done
  • Classification of Epithelial using TJ's: Watch your grammar here ie. “leaky” epithelia on the should have a Capital as is the start of a sentence.
  • TJ Assembly and regulation: Good topic
  • Disease: Very comprehensive Table, don't need the '-' at the end of exhaustive leading into the table. Also images should go in the table if they can it seems like they are just chucked in on the side. Images also need copyright info and a link to the website they came from.
  • Glossary: A few more terms, like JAMs should go in here.


Group 3: Tight Junction

  • Introduction is more like a literature text material.
  • Good history.
  • Structure is good and understandable.
  • Function has a lot of text and makes the reader tired before reading it. OR if you add more photos it will smooth the look of this part.
  • Several diseases are mention and not enough pictures (Pictures are the main speakers).
  • A long table with the diseases, some are left out of it correlated with 2 pictures, not a good idea.
  • Glossary = very short.
  • References need to be uniform
  • The text and the pictures are not balanced properly.

Group 3- Tight Junctions

  • When I look over your project, there is an even amount of text and images.
  • One of the titles of your sections has a spelling error.
  • Desmosomes and hemidesmosomes are part of the same junction type??
  • There are a few spelling and grammar errors but these are easily fixed when you read through, they are fairly easy to see.
  • There is a lot of content. By the end, I am a little tired of reading paragraphs. The tables are a good idea, just to break up the text.
  • Information is easy to access, and very helpful in my understanding.


Tight junctions

  • The history looks quite good, nice and concise
  • For the structure can we get a bit of a general overview? You seem to have gone straight into the specifics. Or maybe if you refer to the picture or something? There just doesn’t seem to be quite enough information to get my head around the precise structure.
  • The ‘molecular components’ section looks really good, well set out and informative, I found it quite readable
  • “barrier function” not “barrier funciton”; subheading in “function”
  • The “function” section was really good, the pictures there correlated nicely with the content
  • Clever integration of tight junction distribution
  • In the “classification of epithelia” section, could you break it up a bit? There is a whole lot of information in one go. Maybe put a few subheading? Dot points? Even another picture or so would help.
  • I really like how you’ve the table for disease. Really well set out, I like the idea of the links, but what exactly are they leading to? When I click on them I get some random NCBI page. Might want to fix that up a bit. If you could like it back to the page where those structures are discussed I think that’d be pretty useful.
  • Some sort of discussion of current research could also be good.


Group 3: Tight Junctions

Introduction And History: The introduction is good due to its concise nature. Also, the history adds to this overview with a good description of how the research into tight junctions has evolved.

Structure: The detailed description of the molecules is helpful, although more about their interaction and placement would be useful.

Classification: Sub-headings in this section, between the types described - tight and leaky - would help to make the section more clear. This was done well in the function section.

Diseases: This is a really clear table showing the diseases, however maybe an extra column with the disease pictures would be more helpful. It would make the relationship between the specific diseases and the adjoining pictures clearer. The introduction to the disease table gives a nice overview and at the same time is very informative.

Glossary: The glossary could be more extensive to assist the reader with quick reference to more of the technical terms.

Workspace

Article:

   * David R. Raleigh,
   * Devin M. Boe,
   * Dan Yu,
   * Christopher R. Weber,
   * Amanda M. Marchiando,
   * Emily M. Bradford,
   * Yingmin Wang,
   * Licheng Wu,
   * Eveline E. Schneeberger,
   * Le Shen,
   * and Jerrold R. Turner

Occludin S408 phosphorylation regulates tight junction protein interactions and barrier function --Robert Dikeman 23:27, 30 March 2011 (EST) Hey guys, here are some possible topics and articles that may be useful for the project

  • Structure of TJs

A lipid-protein hybrid model for tight junction

Molecular Basis of the Core Structure of Tight Junctions

[1]

  • TJs role in disease

Tight junctions: a barrier to the initiation and progression of breast cancer?

  • Formation of TJs

EFA6 Facilitates the Assembly of the Tight Junction by Coordinating an Arf6-dependent and -independent Pathway*


--Mark Hill 17:11, 30 March 2011 (EST) OK Group 3, Just one student trying to organise a discussion group to date and no other content has been added to either your discussion or project page. You were meant to have already begun looking into both the topic and references, pasting links on your discussion page. I will see you in the lab tomorrow to discuss whether you are having problems or are simply not doing the work. This search should get you started. Search Pubmed: Tight Junction


Hey guys..... wanted to try this "discussion" thing...but also wondering if you'll be free to stay back for a little while after the lab on Thursday? --Emma Collyer 13:48, 28 March 2011 (EST)


I actually have a class right after lab. What did you want to talk about?--Robert Dikeman 20:51, 29 March 2011 (EST)


Sorry guys, ive been busy with assignments this week.Anyways,here are some links i found;

Tight junctions and cell polarity

Molecular architecture of tight junctions

Regulation of the movement of solutes across tight junctions

Tight junction pore and leak pathways

I find sirius is pretty handy and resourceful,you guys might wanna check it out.Also,we should break down the task into 4 parts, so each of us is on a part and from that point on , we will mainly do the part we get throughout the session.--Seow Liew 01:54, 31 March 2011 (EST)


Now we're looking better! Awesome articles. Thinking it's important that we have a thorough read through the following to acquire a decent overall understanding & make sure everything we write about is relevant.

Textbooks

Tight Junctions-Molecular Cell biology 4th ed

Oh and keep a sharp eye out for pictures you like. We want to give ourselves as much time as possible to contact the owners if we need permission to use it..

--Emma Collyer 09:10, 31 March 2011 (EST)


Hey Robert, can you post up the potential topics that we might like to discuss on our page here? It'd be best that we have that and maybe base our searches on those topics. Later down the track we might find something else that we might like to add :)

--Paul Lee 08:56, 1 April 2011 (EST)

Hey guys, for the articles that you guys found, could you move them to their respective topic subheadings? Cheers

--Paul Lee 21:08, 1 April 2011 (EST)


Looking good Paul :)... I think we should definitely include a section regarding disease (in humans and maybe other mammals as well?). Then we can talk about it in a commercial context as well.

Tight Junctions and Human Disease I found this article useful!

--Emma Collyer 15:38, 4 April 2011 (EST)


hey guys,I remember Mark mentioned that he wanted us to have a break down on the task and each of us would have to do a part by this thursday.Considering I didnt see any of that , so Ive broken down the task into 4 parts after reviewing the stuff that was on here. Each of us will get to pick a part to do ,just simply put down your signature below the part u`d like to do to indicate it`s taken by you.The last part which is the glossary and reference list is NOT one of the options.Hope you guys cool with this.

Feel free to add any subheading to here if you think of any.


Here are the 4 parts of task break down:

-intro (general idea of tjs), include some pics.

-history (scientists, time frame if possible)


-structure,mainly morphologically and briefly on molecular level.

-types of tjs,leak and tight

-functions or roles of tjs .

--Seow Liew 02:36, 5 April 2011 (EST)


-components of the tj; common proteins that are found associated with tjs,categories and what they do( eg. proteins that contribute to the formation of tight junctions, paracellular transport) and solute gradients within the tight junction,if there`s any of them.

-transepithelial transport -paracellular transport, how does it work and components involved. (other types of transport if there`s any more)


-discuss briefly on tjs related current research ( newly found tj interacting proteins , molecules and how are they related to the junction)

-types of disease that are related to tjs.- Emma



glossary reference list


--Seow Liew 16:41, 6 April 2011 (EST)



IMPORTANT

Hi guys, for the sake of order on this page, lets put our progess on the "work space" and leave lines between each post. Also, Desmond, in regards to your subheadings, isnt "-common proteins that are found associated with tjs,categories and what they do" related to the structure of the TJ? I feel we need to finalise on the topics we will be discussing before we allocate our tasks. Meet up on wednesday? :( i lost my sheet with all our emails on it....so this is my only form of communication with you guys...assuming you guys dont use your uni email...

--Paul Lee 15:48, 5 April 2011 (EST)

yes , it also is.Those proteins serve 2 purposes , establishing tight junctions and facilitating paracellular transport.In part 1, under structure ,I thought we`d talk mainly on the morphological structure , and brief through molecular components of it considering we`re having an exclusive section for proteins and other molecules that are associated with tjs.I did some editing on certain parts , just to make it clearer. --Seow Liew 16:44, 6 April 2011 (EST)





If you don't mind I'd like to do the section on diseases .... and then I'll do whatever else is left over :) --Emma Collyer 12:16, 6 April 2011 (EST)

Hey Emma ,I left out a detail that I thought it should be included in your part, would you mind doing -the significance or important applications of tjs as well? Here`s a link related to it. [2] --Seow Liew 16:41, 6 April 2011 (EST)

Hey guys, these topics look really good. I would be happy to do any of them really. i think it might be easier to pick them in person though. Let's talk tomorrow after lab --Robert Dikeman 22:17, 6 April 2011 (EST)



I can absolutely do that! As we discussed today, we can refine topics, headings and subheadings once we each have a general research area :)

--Emma Collyer 23:33, 6 April 2011 (EST)



Hey Guys, for the historical background...do you know of any sites or anything that may be of use. I'm coming up empty :(

--Paul Lee 23:47, 7 April 2011 (EST)


Hey Rob, this is the link- http://www.ncbi.nlm.nih.gov/pubmed/9558458

also remember: paracellular transport + transcellular transport :)

--Paul Lee 09:30, 5 May 2011 (EST)


Hey emma,

I found stuff on Gliadin (from wheat) and Zonulin (released from intestinal cells) and they seem to be proteins that arent components of the TJ structure............

Gliadin, the main fraction of wheat gluten responsible for the intestinal damage typical of celiac disease (CD), is the environmental factor that triggers this disorder [1]. It is known that CD is the result of an inappropriate T-cell-mediated immune response against ingested gliadin [2]. CD is associated with the HLA alleles DQA1*0501/DQB1*0201, and in the continued presence of gliadin the disease is self-perpetuating [3]. One of the autoimmune targets of CD is tissue transglutaminase (TTG) [4]. The deamidating activity of this enzyme generates gliadin peptide fragments that bind to DQ2 and to DQ8 so as to be recognized by disease-specific intestinal T cells [5]. This process activates a cascade of events in which cytokines and matrix metalloproteinases are up-regulated and the intestinal mucosa is destroyed [6], [7]. CD is currently regarded as a paradigm of autoimmune disease for which the main genetic predisposition (HLA-DQ2/DQ8), the exogenous trigger (gluten), and one of the autoantigens (TTG) are known. In recent years much has been discovered about the genetic and immunologic aspects of CD [2]. However, little is know about the possible interactions of gliadin (and/or its peptide derivatives) with intestinal epithelia and the mechanism(s) through which it crosses the epithelial barrier to reach the submucosa. Under physiological circumstances, intestinal epithelia are almost impermeable to macromolecules such as gliadin [8]. Several studies reported that CD is a condition in which paracellular permeability is enhanced and the integrity of the tight junction (tj) system is compromised [9], [10]. The up-regulation of zonulin, a recently described intestinal peptide involved in tj regulation [11], seems to be responsible, at least in part, for the increased gut permeability characteristic of CD [12]. Following stimulation of normal rat intestinal cells (IEC6) with gliadin, zonulin is released and induces a protein kinase C-mediated polymerization of intracellular actin filaments, which are directly connected to tj complex proteins, thereby regulating epithelial permeability [13]. Furthermore, the persistent presence of inflammatory mediators such as tumor necrosis factor-α (TNF-α) and interferon-γ (INF-γ) have been shown to increase the permeability across the endothelial and epithelial layers [14], [15].

Read More: http://informahealthcare.com/doi/full/10.1080/00365520500235334


http://en.wikipedia.org/wiki/Help:Table

Header 1 Header 2 Header 3
row 1, cell 1 row 1, cell 2 row 1, cell 3
row 2, cell 1 row 2, cell 2 row 2, cell 3
row 3, cell 1 row 3, cell 2 row 3, cell 3

Hey guys, really bad news :*( my computer died on me...and ive lost alot of the stuff i was gonna put up...im actually quite sad. :(:( ill try remember all that ive done but i dunno...currently at uni atm ...so try do as much as i can before i get home :( :( :S(oasidhosahdoisa im really sad right now... :(

--Paul Lee 18:17, 11 May 2011 (EST)


Homework

31 March 2011

Review articles-

Physiology and Function of the Tight Junction

Molecular Basis of the Core Structure of Tight Junctions

Molecular complexity of vertebrate tight junctions

Research articles-

2011- Imaging analysis of the gliadin direct effect on tight junctions in an in vitro three-dimensional Lovo cell line culture system

1969- Freeze-etch appearance of the tight junctions in the epithelium of small and large intestine of mice

The title sums it up pretty well- really just structural observations of tight junctions in freeze-etched epithelial cells the jejunum and colonin mice!

I'll write summaries for each later today! --Emma Collyer 10:51, 31 March 2011 (EST)




Research articles

2011 - Tight Junction Zonula Occludens-3 regulates Cyclin D1 dependent cell proliferation.

This is just some information on TJ's and their role in the regulation of cell proliferation. Seems like it could be used as a means for regulating cell proliferation in carcinogenesis

1998 - The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein.

this article deals with the relationship between tight junctions and gap junctions. Might be interesting to add to our page?

2007 - Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's disease.

This article is about Crohn's disease (an inflammatory disease of the intestines) and the attempt to understand the mechanisms (paying special attention to TJ's)

Review Articles

1998 - Role of tight junctions in establishing and maintaining cell polarity.

This article is just outlining the role of tight junctions, the importance in maintaining cell polarity.

--Paul Lee 10:57, 31 March 2011 (EST)


Review article:

The tight junction: morphology to molecules

-This article is about the types of proteins and other molecules that are associated with tight junctions and the roles they serve.

Current research article: (2011)

A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells

-This article talks about how protein ZO-1 acts as a junction interacting protein which helps with recruiting paracingulin to tight junctions.

Historic research article: (2002)

Regulation of Airway Tight Junctions by Proinflammatory Cytokines

-This article suggests that the TJ of airway epithelia exposed to chronic inflammation may exhibit parallel changes in the barrier function to both solutes and ions.

--Seow Liew 23:54, 4 April 2011 (EST)



Review Article:

Fluid transport across leaky epithelia: central role of the tight junction and supporting role of aquaporins

This article concerns the role of tight junctions in transport across the epithelia.

Research Article:

(2011)

Tight Junctions and Tissue Barriers

This article shows the role of tight junctions in tissue structure and function.

(1971)

Junctions between cancer cells in culture: ultrastructure and permeability

This article is about the differences in cell junction composition between cancer and normal cells. This may be good to see the role that tight junctions (or lack there off, in this case) is involved in cancer.

--Robert Dikeman 21:57, 6 April 2011 (EST)

General outline of our topics

Tight Junctions

Introduction

Dr Hill just showed me something cool! Obviously we need to include the proteins in tight junctions....and if you go to pubmed and search the protein name under all databases, it comes up with lots of great information. If you click on OMIM there is a summary of important information. He suggested that we include links to these pages for each of these proteins so that the information is easily obtainable. Sounds like a really good idea....obviously this can go in structure if you think it's more appropriate

--Emma Collyer 11:31, 7 April 2011 (EST)



History

Structure

Molecular components

Roles

Role of tight junctions in establishing and maintaining cell polarity.

Junctional Complexes in Various Epithelia

Tight Junctions/Adherens Junctions: Basic Structure and Function

Tight junctions are crucial to the structure and function of individual epithelial cells as well as the epithelium as a whole. One of the major roles of the junction the joining together of the plasma membranes of adjacent epithelial cells to form a tight seal that prevents the free diffusion of material across the epithelium via the inter-cellular space. This serves the crucial function of maintaining the differences in chemical composition of one side of an epithelial sheet versus the other. The creation of these two structural compartments is essential for the proper function of epithelial tissue. This is very important in the intestine for example, which must be able to regulate which substances pass from the intestinal lumen into the bloodstream, as well as when this transport should occur. (add other examples, will talk about more in next section). Another major role of the tight junction is separation of the plasma membrane of individual epithelial cells into apical and basolateral regions. (how does it do this? how is trans TJ diffusion restricted?) This allows for the localization of specific transmembrane proteins that may only be functional in a particular location of the membrane (give examples and more explained why)...

Transepithelial Transport

Maintaining Intracellular Polarity

Maintaining Plasma Membrane Polarity

TJ Formation Regulation

Classification

Current associated research

Tight Junction Zonula Occludens-3 regulates Cyclin D1 dependent cell proliferation.

Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's disease.


Very few patents exist for compounds that claim to treat TJ malfunction, and most of the existing patents are based in the United States. The specifications for some of these patents can be found below-

WO 1998/005359

WO/2009/006246

WO/2008/113030

WO/2010/110210


Diseases

Modulation of TJ barrier function in human disease


Astrocyte–endothelial interactions at the blood–brain barrier

Astrocyte–endothelial interactions at the blood–brain barrier 2

Important applications

Glossary

Further Information

Reference list