Talk:2010 Lecture 3
- A marker for sequential exocytosis The SNARE protein SNAP25, say Takahashi et al., marks the plasma membrane after an initial exocytic event to allow rapid sequential exocytic events.
- A myosin V moves yeast secretory vesicles Secretory vesicles actively move to the site of exocytosis in yeast. Schott et al. find that multiple secretory vesicles often follow the same linear track and frequently enter and cross the bud. This movement requires the activity of the myosin-V heavy chain encoded by the MYO2 gene. When the predicted lever arm of this motor is progressively shortened (with the most extreme example being the 0IQ mutant), the vesicle movements are progressively slowed.
- Rapid cycling of lipid rafts to and from the Golgi Nichols et al. detect rapid cycling of lipid raft markers between the plasma membrane and the Golgi. Through selective photobleaching, they are able to study transport either out from the Golgi to the plasma membrane, or in from the plasma membrane to the Golgi.
- Membrane docking at the immunological synapse requires Rab27a Stinchcombe et al. find that normal membrane docking of lytic granules at the immunological synapse is defective in cells lacking Rab27a. In cells lacking other Rab proteins, polarization of the secretory granules is incomplete.
- Visualizing the location and dynamics of exocytosis Schmoranzer et al. use total internal reflection (TIR) fluorescence microscopy to visualize exocytosis in mammalian cells (e.g., see event on left side of video). The analysis reveals that there are no preferred sites for constitutive exocytosis in this system.
- Visualizing the location and dynamics of exocytosis Toomre et al. use a combination of TIR microscopy (green, labeling molecules close to or at the membrane) and standard fluorescence microscopy (red, for molecules further from the membrane) to visualize [/content/vol149/issue1/images/data/33/DC1/Fig_1b.mov trafficking to and fusion with] the plasma membrane during exocytosis. Red dots turn yellow then green as they approach the membrane, and then explode in a burst of light as they fuse with the plasma membrane during exocytosis. The transport containers appear to be partially anchored at the membrane before fusion, and can undergo either partial or complete fusion events.
- Journal of Cell Biology (JCB) copyright notice extract "Beginning six months after publication, Rockefeller University Press grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode."
- File:Membrane label and endosomes.mov
- Dynamic Changes in the Spatiotemporal Localization of Rab21 in Live RAW264 Cells during Macropinocytosis
- Ordered Patterns of Cell Shape and Orientational Correlation during Spontaneous Cell Migration
- EM image of Actin cytoskeleton
- Automated Builder and Database of Protein/Membrane Complexes for Molecular Dynamics Simulations
- Comparative Dynamics of Retrograde Actin Flow and Focal Adhesions: Formation of Nascent Adhesions Triggers Transition from Fast to Slow Flow
- Properties of the Force Exerted by Filopodia and Lamellipodia and the Involvement of Cytoskeletal Components
- Golgi Cisternal Unstacking Stimulates COPI Vesicle Budding and Protein Transport
- Radial Glial Dependent and Independent Dynamics of Interneuronal Migration in the Developing Cerebral Cortex
- Heparanase Facilitates Cell Adhesion and Spreading by Clustering of Cell Surface Heparan Sulfate Proteoglycans
- Live Imaging at the Onset of Cortical Neurogenesis Reveals Differential Appearance of the Neuronal Phenotype in Apical versus Basal Progenitor Progeny
- Fusion of the Endoplasmic Reticulum and Mitochondrial Outer Membrane in Rats Brown Adipose Tissue: Activation of Thermogenesis by Ca2+
- Flotillins Interact with PSGL-1 in Neutrophils and, upon Stimulation, Rapidly Organize into Membrane Domains Subsequently Accumulating in the Uropod
- Septins Regulate Bacterial Entry into Host Cell Septins are conserved GTPases that form filaments and are required in many organisms for several processes including cytokinesis.
- BH3 Peptides Induce Mitochondrial Fission and Cell Death Independent of BAX/BAK Mitochondria Fission
- Asymmetry of Early Endosome Distribution in C. elegans Embryos Endosomes (single movie)
- Scyl1 Regulates Golgi Morphology Golgi EM and movies
- Tubular Bridges for Bronchial Epithelial Cell Migration and Communication Membrane structures
The following material is a draft notebook area, not part of the current lecture content.
- Vertebrate membrane proteins: structure, function, and insights from biophysical approaches. Müller DJ, Wu N, Palczewski K. Pharmacol Rev. 2008 Mar;60(1):43-78. Epub 2008 Mar 5. Review. PMID: 18321962
- The Cell - Association of Actin Filaments with the Plasma Membrane http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=plasma%20membrane&rid=cooper.section.1764#1778
- Molecular Biology of the Cell Figure 10-9. Cholesterol in a lipid bilayer
- Cell motility through plasma membrane blebbing Oliver T. Fackler and Robert Grosse J Cell Biol. 2008 June 16; 181(6): 879–884. doi: 10.1083/jcb.200802081. PMCID: PMC2426937
- UNSW Researchers - http://jcb.rupress.org/cgi/content/full/171/1/121?
- Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography. Morone N, Fujiwara T, Murase K, Kasai RS, Ike H, Yuasa S, Usukura J, Kusumi A. J Cell Biol. 2006 Sep 11;174(6):851-62. Epub 2006 Sep 5. PMID: 1695434 JCB http://jcb.rupress.org/cgi/content/full/174/6/851?