Talk:2009 Group 2 Project

From CellBiology

Group Peer Review

A. This page is a well organised page. It provides comprehensive information on the topic of Apoptosis, which shows extensive research skills. The hand-drawn diagram is complementary to the information provided, NICE WORK! The clinical view section gives a more in depth understanding of the topic, with examples of various diseases.

However, you may consider the following suggestions for improvement of the page. First of all, the overall format can be more consistent. The subheadings from the sections of introduction to signaling were bold, yet onwards from regulation of apoptosis appear to be italic. Secondly, there has not been much information on current research of the topic. Thirdly, the section of definition can be incorporated into the introduction section. Sections such as why does apoptosis occur can also be divided into further subheadings for easier understanding.

B. The first thing that hit me when I viewed this page was the calibre of the layout. It is really well set - out, the subheadings are concise and draw the reader in, I think this is largely due to the fact that some the headings themselves are questions.

  • The timeline, was a good overview of the major events that have occurred in the subject area.
  • The referencing was in the correct 'wiki' format. Congratulations on that as it is quite arduous to do.
  • The inclusion of various pathways solidified our understanding of the process of apoptosis. It is clear to us that there has been significant research conducted to create this group project.
  • The inclusion of a wide variety of beneficial pictorial aids was another highlight of this page. They were all approporiately labelled and referenced.
  • There wasn't anything obvious that needed alteration. Well done guys!!

Uploading Errors

I made a image file upload without placing in the z in front of the file. The file is Apoptosis_Pathway.JPG

Timeline for next 3 weeks

  • 7/5 - 14/5
    • Swapping sections; analyse and interpret
      • Nathan <--> Min
      • Beatrix --> Jessica
      • Jessica --> Daniel
      • Daniel --> Beatrix
    • Individual work on own section
      • Finish all diagrams
      • Summarise important information into tables or flow diagrams
      • Reduce irrelevant rambles into concise points
    • Note; all changes to be made on discussion, not main page
  • 14/5 - 21/5
    • Everyone to read through entire page
      • Make sure nothing is repeated
      • Logical flow
      • Any adjustments to be made on discussion first
  • 21/5 - 19/5
    • Arrange a time to meet to analyse entire project together


--Daniel Lee 19:33, 31 May 2009 (EST) hey guys I've rearranged the referencing order so that it looks neat, can you guys add in your missing glossary, I've already done my part its just yours that needs to be included, please add it in before the hand it date cheers

--Daniel Lee 11:34, 27 May 2009 (EST) guys remember to make a internal link which links back to your individual project and vice versa

--Daniel Lee 01:39, 27 May 2009 (EST) hi guys, I've just re-do the layout of my section - so that it doesn't look to lengthy, oh yeah, guys remember to add some photos before the due in date. Cheers

--Jessica Lazarus 00:43, 27 May 2009 (EST) Hey guys. I've completed my section for the group project. The reference list is quite substantial because of the history section. Anyway, I hope u like =]

--Jessica Lazarus 23:01, 24 May 2009 (EST) Hey guys. I've added more to my section. If you like you can read it now or when I'm done with the history part. As far as I can tell it doesn't look like anything has been repeated. I read the other sections as well. Good job. Looks really good. Cheers.

--Min-Soo Kim 18:26, 18 May 2009 (EST) Hey guys, I've deleted my part of abnormalities as it was overlapping with Nathan's work. I'll focus more on my regulations of apoptosis area. Cheers

--Min-Soo Kim 20:45, 14 May 2009 (EST)Hey guys, I've added Regulations of the apoptosis part, although this is draft and still more to be added. At the moment it has little bit of repetition of the info with apoptosis signalling pathway, I'll try to fix and delete any part which might be a repetition. Cheers~

--Nathan Holloway 10:20, 14 May 2009 (EST) hi guys, I won't be coming in today, under the weather. I'll read over what you post and do watever needs to be done. Sorry guys

--Min-Soo Kim 04:50, 14 May 2009 (EST) Hey guys, I've just realized that we are missing the antiapoptotic mechanisms of apoptosis and regulatory proteins of apoptosis. As we talked about the major pathways of apoptosis :mitochondra-dependent pathway, and Death receptor-mediated pathway etc, I think we should talk about the antiapoptotic mechanisms which regulate(inhibit apoptosis) the pathway such as Bcl-2 family proteins govern mitochondra-dependent pathway, ADED family proteins prevent initiaor caspase activation, IAPs family bind to and inhibit active effector caspases etc.. As I have significant amount of topic overlap with Nathan, I can do this part if you guys don't mind. Or Is Jessica doing this part? Cheers~

  • Task Allocation

--Min-Soo Kim 05:37, 5 May 2009 (EST) Hey guys I've added draft of my work, I think I needs a lot of more work to do. I'll keep working on it in this week. And I've chosen Bcl-2 proteins for my individual project.

Hey guys in-regards to the thursday lab, I think that Mark wants us - as a group to post information up, even though it is still in its drafting stage, so Min Soo can you post your draft copy for the Abnormality part, Jessica can you do the History of Apoptosis - you can consider doing it in point form, Beatrix & me will will post up the images and tables, I think it should apply to everyone aswell, just post up images or do internal links to website cheers --Daniel Lee 15:32, 1 May 2009 (EST)

--Daniel Lee 16:38, 26 April 2009 (EST)Hey guys I re-do my part again for the Developmental Morphology, I added in the morphology for necrosis aswell, I think my introduction doesn't sound right please give advice

--Beatrix Palmer 08:33, 21 April 2009 (EST) hey guys i added my section last week..i think its still a little bit rough so i need to edit it. im also in the process of drawing up a diagram. see you in class. --Daniel Lee 11:39, 14 April 2009 (EST) For my individual project I've choosen TUMOR PROTEIN p53 - I just want to make sure that I don't clash with anyone cheers

--Daniel Lee 19:09, 13 April 2009 (EST)I've redo my part again, I just went over some mistakes which I've found and added in some addition info, I'm still missing the last stage of apoptosis and will fix that part later this week

--Mark Hill 00:02, 13 April 2009 (EST) Your group is working well here, keep it up. Remember that you can paste any resources you may need here to share with the group, including key references/reviews and images.

--Nathan Holloway 11:37, 10 April 2009 (EST) Hey guys, I've posted my part, I know it's quite long and needs to be heavily reduced. I covered a bit of abnormalities as well, thought I'd include it in my section so Min can check it out and see if he wants to use any of it. Hope it's alright. enjoy your easter break

--Jessica Lazarus 00:17, 10 April 2009 (EST)Hey guys just letting you know that I'm going to do TNF for the individual project. Must we all have a different protein? --Mark Hill 00:01, 13 April 2009 (EST) Yes.

--Beatrix Palmer 12:52, 9 April 2009 (EST) i have chosen Apoptosis Inducing Factor (AIF) for my individual report. just to let you all know so we don't clash. cheers Bea. --Nathan Holloway 10:32, 9 April 2009 (EST) Hey guys, I can't make it into lab today as I have a religous holiday today. I have done all my research I'm just typing it all up. I'll have it up by tomorrow. Enjoy class!!

--Beatrix Palmer 14:57, 8 April 2009 (EST) Hey Guys. Daniel, i really think we should double check to see if we are going to overlap our info. ill bring in a rough draft tomorrow. i think i will need to put in a diagram as well... so if you just want to stick to a diagram for your section, we can all put a full timeline together towards the end??? --Nathan Holloway 19:00, 7 April 2009 (EST) Yeah I have no problem with that. I also think it's a good idea to include the inhibitors, and if it's too much we can always reduce it later

Its Daniel here, can I wait for Beatrix to hand in her part before I do the timeline, I'm not quite sure whether I should only do the morphological aspect for the timeline - should I include the main inhibitors aswell.

Outcomes of the Group Meeting: Project Outline:

Jessica: Introduction: to all topics and breif explanation of what, where, how and issues associated with Apoptosis. Conclusion: A summary of what was talked about and what it all means for the future.

Beatrix: Apoptosis Signalling: The role of caspases, adaptor proteins, TNF, Bcl-2 family in Apoptosis.

Daniel: Developmental Stages & Morphology: processes involved in the initiation of Apoptosis (including morphological changes) and the various stages in ivolved in Apoptosis including the induction and inhibition of Apoptosis.

Min Soo: Abnormalities: the various abnormalities associated with Apoptosis and disruptions to the processes of Programmed Cell Death, including genetic abnormalities and their associated pathologies.

Nathan: Current Research & The Future: covering current and new finding associated with Apoptosis and their potential clinical implications and the significance of the research, as well as the future direction of this research.

Meeting: 1-2pm Wednesday 1st April, in Rm 106.

Allocation of topics

  • Intro- Structure & dynamic Function (Jess)
  • Developmental Process/Signalling (Bea and Daniel)
  • Timeline (Daniel)
  • one student-designed figure illustrating cell-death
  • current research about cell-death (Nathan)
  • Abnormalities (Min)


--Jessica Lazarus 12:01, 26 March 2009 (EST) Yeah that sounds good. Im free on monday and friday sounds good too. Should be fun! Ciao. Jess.

--Min-soo Kim 00:47, 26 March 2009 (EST ) hello, I'm Min, Yeah let's organize a time so that we can discuss about our topic. I'm free all day on monday and friday.

--Beatrix Palmer 14:36, 25 March 2009 (EST) hey lets organise a time in the lab tomorrow to go over our topic. have a think about when would work for you. im free all day monday, tuesday arvos and the middle of the day on fridays.

--Daniel Lee 18:19, 21 March 2009 (EST) Hello Group 2, I am Daniel Lee, I've read some information in regards to Apoptosis, in which, the mechanisms that regulates cellular death illustrates both structural and functional events and so I just want to ask whether you guys want to talk about the intercellular signal or p53 tumour suppressor protein which regulates the apoptosis.

--Mark Hill 14:01, 19 March 2009 (EST) I have added a link to all group projects please leave it at the bottom of your project page.

I have also moved your Microscopy work here.

Electron Microscope

Home Page of Electron Microscope

The Electron Microscope Unit contains an variety of facilities, in which, the following equipment are

  • Phillips CM200

The Philips CM200 field emission gun transmission electron microscope allows very high resolution images to be obtained from thin, electron transparent sections of materials.

  • JEOL 1400

The JEOL 1400 transmission electron microscope is principally used in the analysis of thin sections of both biological materials and polymers.

  • Hitachi S900

The Hitachi S-900 is an 'in-lens' field emission scanning electron microscope capable of imaging at very high resolutions (~0.5nm).

  • Hitachi S4500

The Hitachi 4500II is a "below lens", field emission scanning electron microscope. It is used for microstructural analysis of a very wide range of materials and is capable of very high resolution (~2nm) images.

  • FEI Quanta 200 ESEM

The FEI Quanta 200 is a particularly versatile microscope capable of operating in three different vacuum modes. It may be used in a high vacuum or conventional mode to examine dry conductive specimens.

  • Hitachi S3400

The UNSW EMU has two Hitachi S3400 SEMs – the S3400-I (for imaging only) and the S3400-X (for imaging and X-ray microanalysis).

  • XT Nova Nanolab 200

The xT Nova NanoLab 200 combines a dual beam high resolution focussed ion beam (Ga FIB) and a high resolution scanning electron microscope.

  • Focused Ion Beam XP200

The FEI XP200 focused ion beam miller uses a fine, energetic beam of gallium ions that scan over the surface of a specimen.

  • Digital Instruments 3000 AFM

The atomic force microscope (AFM) probes the surface of the sample with a sharp tip that is a couple of microns long and around 10 – 60nm in diameter.

  • Digital Instruments Multimode

The Multimode atomic force microscope (MM-AFM) is designed for imaging small samples (15mm diameter or less).

  • JEOL JXA-8500F Field-Emission SEM-EPMA Hyperprobe (WDS & SDD-EDS)

The JEOL JXA-8500F is a powerful tool in the microanalysis of materials.

  • Skyscan 1072

The SkyScan 1072 computerised x-ray tomograph comprises an 83kV x-ray sealed microfocus source with a spot size of less than 8µm, an x-ray CCD camera with a resolution of 1024x1024.