Melanoma Chemotaxis Movie
This movie shows how cells can respond to a growth factor gradient by migrating towards the source (higher concentration) of the chemotactic agent.
MV3 cells migrating over the narrow bridge of the Insall chamber in the presence of a 10% fetal bovine serum (FBS) gradient to the right.
The images were taken every 5 minutes for 12 hours using a 10x phase objective.
|Increasing concentration Fetal Bovine Serum ->|
Andrew J Muinonen-Martin, Douwe M Veltman, Gabriela Kalna, Robert H Insall An improved chamber for direct visualisation of chemotaxis. PLoS ONE: 2010, 5(12);e15309 PubMed 21179457
Copyright: © 2010 Muinonen-Martin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Muinonen-Martin AJ, Veltman DM, Kalna G, Insall RH (2010) An Improved Chamber for Direct Visualisation of Chemotaxis. PLoS ONE 5(12): e15309. doi:10.1371/journal.pone.0015309
Original file name: Journal.pone.0015309.s002.mov
Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G