Independent Learning Projects

From CellBiology

Introduction

This current page is a link to Independent Learning Projects carried out in the Cell Biology Laboratory.

The purpose of this online format is for students to maintain documentation of their project's progress in addition to their own laboratory notes, experimental analysis and supervisor meetings. Please note that this is an internet page viewable by anyone and information should be carefully assessed prior to being added.

Projects

  • 2009 ILP Neural
    • ILP z3221319 Development of a Retinal Ganglion Model for Glaucoma


Did you know that when you have logged in, "my preferences" at the top of the screen allows you to be notified of changes to your ILP Talk page?

  1. Select "my preferences" at the top of the page.
  2. Under the heading E-mail select the box for "E-mail me when a page on my watchlist is changed".
  3. Now go to your ILP project talk page and just click the tab once at the top of the screen it will change from watch to unwatch.

You will now get an email to your specified account whenever a change has been made to your talk page, like a comment from your supervisor.... This can be turned off at anytime by clicking unwatch and it is an option for all the webpages.

ILP Online Questions

Is this examinable?

  • No

What should I put online?

Begin with this.....

  • The project hypothesis and aim.
  • Having prepared your literature review you may wish to include text or excerpts from your written document.
  • A list of background/related references and links.
  • Research protocols/methods.
  • Experimental write ups.
  • Anything you consider relevant to your project.

Do I have just 1 page?

  • You can have as many pages as you think you require for your project.
    • each page title must begin with your student number.
  • You may want to have separate pages for each experiment in your project.
    • Perhaps with a summary of results on your first page.
  • You may want a page with references structured into reading groups.
    • or a summary of what the paper shows.

Who can see my page?

  • Anyone who has internet access can view your project pages.
  • Therefore avoid specifically identifying yourself (unless you want to). I would prefer you to leave your my preferences settings as your student number.

Who can edit my page?

  • You can edit your own page from any computer. Editing Basics
  • Alternatively for tables and easy formatting use Open Office and export as Wiki format.
    • This will make a text file you can copy and paste into your page.
  • Only students, staff and researchers given access to the Cell Biology Wiki.
  • There is no anonymous editing.
    • All page edits are logged by user and can be checked using "history" tab at the top of the page.

Where can I post my draft work?

  • Use the "discussion" tab at the top of the page.
    • This area can be used like a notepad and your supervisor can also leave notes on this page.

How can I track my work?

  • You can signature stamp new content in "edit mode" the small icon "Your signature with timestamp" at the beginning of your entry.
  • You can also be notified of changes to your ILP Talk page (or any page).
    • Select "my preferences" at the top of the page.
    • Under the heading E-mail select the box for "E-mail me when a page on my watchlist is changed".
    • Now go to your ILP project talk page and just click the tab once at the top of the screen it will change from watch to unwatch.

You will now get an email to your specified account whenever a change has been made to your talk page, like a comment from your supervisor.... This can be turned off at anytime by clicking unwatch and it is an option for all the webpages.

How can I upload research images?

  • Use the "Upload file" option located near the bottom of the left hand menu.
  • Please note all images you upload to this site must begin with the letter Z
  • Image must have a descriptive name related to its content
    • if the name already exists the site will show you the other image, just rename your image
  • All images uploaded must contain their original source in the text box, a link to the reference source and a copy of the copyright information
  • With your own research images it is important to accurately represent your research data.

Rossner M, Yamada KM. What's in a picture? The temptation of image manipulation. J Cell Biol. 2004 PMID: 15240566

Laboratory Induction

Before you can complete a laboratory OHS Induction, you will need to have completed UNSW Safety training courses.

Cell Biology Lab - Health and Safety

UNSW HS Courses

  • Appropriate training is required before laboratory induction and experimental work can commence.
  • Training required is outlined in the document SOMS Training descriptions PDF

In Cell Biology Laboratory the following courses are currently applicable to different projects.

All courses require registration and the current timetable shows course availability.

UNSW Links: OHS Training Courses | OHS Training Courses

Laboratory Induction

  • The laboratory induction process will take approximately 30 - 45 minutes.
    • Appropriate laboratory clothing and closed toe shoes should be worn.
  • You will need additional time following induction to become familiar with specific OHS safety issues.
  • The first time any laboratory procedures are undertaken should be directly supervised.
  • Please ensure your laboratory manual has written protocols and procedures that you will follow for your experiments.
  • Your laboratory induction will work through the form check list OHS induction checklist for academic supervisors of research students (PDF - 93KB)
  • On completion of the induction both student and supervisor will sign and you should retain a copy of the form with your ILP documents.
  • The OHS links below are to specific resources that you should read or use as part of your follow up to induction training.

OHS Links: OHS induction checklist for academic supervisors of research students (PDF - 93KB) | SOMS OHS | Evacuation Map | Emergency Personnel | OHS Training | Safe Working Procedures | Risk Assessments | Acts and Standards | Policies, Guidelines and Procedures | Chem Alert

PC2 OGTR Laboratory

  • Before commencing work in the SOMS PC2 OGTR Laboratory you will need to complete the appropriate UNSW OHS Courses and the Laboratory induction.
  • All work in the laboratory needs to be entered in the PC2 Laboratory Log Book. Autoclaving of all biological waste also requires inclusion of monitor and entry in the PC2 Laboratory Log Book.
    • PC2 Laboratory Log Book is the blue diary located on the bench with computers.
  • For tissue culture work the class 2 Biohoods need to be booked using the BioHood Booking Calendar
  • The current SOMS Biological Safety Supervisor is Dr Gilles Guillemin.

PC2 Links: PC2 OGTR Lab Information | SOMS Biological Safety Supervisor | BioHood Booking Calendar

Chemicals (MSDS)

  • I have prepared a separate page ILP MSDS where you should list links to all chemicals you potentially may use in your current project.
  • UNSW subscribes to the Chem Alert database. Use your student ID and Unipass for log-in.
  • There are several different additional MSDS databases available. Please note that some information may differ from country to country.

MSDS Links: Chem Alert | USA PubChem

Universal Precautions

  • When dealing with biological materials, in particular human specimens, are a set of precautions designed to prevent transmission of human immunodeficiency virus (HIV), hepatitis B virus (HBV), and other bloodborne pathogens when providing first aid or health care. These precautions should also be used when carrying out basic research on these tissues.
  • Universal precautions involve the use of protective barriers (PPE, personal protective equipment) such as gloves, gowns, aprons, masks, or protective eyewear, which can reduce the risk of exposure of the health care worker's skin or mucous membranes to potentially infective materials. In addition, under universal precautions, it is recommended that all health care workers take precautions to prevent injuries caused by needles, scalpels, and other sharp instruments or devices.


Links: CDC Universal Precautions for Prevention of Transmission of HIV and Other Bloodborne Infections

Health and Safety (HS)

  • School of Medical Sciences, Health and Safety (HS) Committee
    • "To facilitate a safe work environment by developing and documenting HS programs to coordinate training of staff and students and by overseeing the implementation of HS procedures and policies in the School of Medical Sciences."
  • OHS - Australian Acts and Standards | Australian Acts and Standards
  • An HS Management System (HSMS) is a set of plans, actions and procedures to systematically manage health and safety in the workplace that is actively endorsed by a committed employer to achieve:
    • Provision of a safe and health workplace and the prevention/reduction of illness and injury equally for employees and contractors.
    • Identification of workplace hazards, assessment and control of all risks.
    • Active involvement in health and safety matters by managers, supervisors and employees and their representatives.
    • Provision of information and training for employees at all levels so they can work safely.
    • Audit and review of the HSMS.
    • UNSW HS Management System

Methods

Links to some relevant laboratory experimental protocols. See also Chemical MSDS Links

Tissue Culture

Molecular Biology

Immunochemistry

Links

Internal Links

External Links