ILP Tissue Culture

From CellBiology

Tissue Culture Media

There are a range of different media formulations suitable for mammalian cell culture. Commonly used media formulations are shown on the Tissue Culture Media page.

  • Dulbecco's Modified Eagle Media - available in high and low glucose formulations, with and without phenol red and other components.


Media Supplements

Serum

  • Fetal Calf Serum
  • Horse Serum

Penicillin-Streptomycin

  • Contains 10,000 units of penicillin (base) and 10,000 µg of streptomycin (base)⁄ml utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline.
  • Spectrum: Gram-positive and gram-negative bacteria.
  • Recommended concentration: 50 to 100 U of penicillin, 50-100 µg of streptomycin.
  • Invitrogen | MSDS

Growth Factors

Biological Waste

  • Biological Waste should be taped closed in the bin.
  • An indicator taped to the bag (1 /load)
  • Transported double contained to the autoclave for sterilization.
  • Treated waste should then be transferred to the SOMS waste (LG floor).
  • A completed OHS Biological Waste form should then be Faxed to 9385 2365.

Links: OHS Waste - Laboratory Hazardous Waste Disposal | OHS Biological Waste form PDF


Suppliers

Media

Invitrogen

Invitrogen Australia Pty Limited Level 3, 501 Blackburn Road Mount Waverley VIC 3149 Australia Orders: 1800 331 627 Techline: 1-800-009-887 Phone: (61-3) 8542-7400 FAX: (61-3) 9544-5622 Email: mail.australasia@invitrogen.com

Glass Coverslips

ProSciTech Cat.No. G402 coverslip #1, 13 mm diameter http://www.proscitech.com/cataloguex/online.asp?page=g2#

ProSciTech PO Box 111 Thuringowa Central Queensland 4817 P: +617 7443 9444 http://www.proscitech.com/