File:PMID11564755-Nuclear pore complexes04.mp4

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PMID11564755-Nuclear_pore_complexes04.mp4(file size: 3.92 MB, MIME type: video/mp4)

Nuclear Pore Complexes

In vivo analysis of GFP3-hNup133 and GFP3-hNup107 dynamics in mitosis. (A) Four-dimensional confocal sequence of an NRK cell stably expressing GFP3-hNup133. The sequence extends from metaphase/anaphase transition to telophase. Confocal z-stacks with six slices every 1.5 μm were acquired in 5-s intervals. A single z-slice containing the best-focused GFP signal is displayed for representative time points. Left panels show GFP fluorescence to localize the nucleoporin; right panels show the simultaneously acquired DIC images to visualize the chromosomes. Due to the low signal to noise ratio in a single confocal section, fluorescence images were contrast enhanced and smoothed with a 3 × 3 median filter to remove detector shot noise.

(B) Same as in A but from a cell stably expressing GFP3-hNup107. Time interval between z-stacks is 10 s. Note the earlier diffuse recruitment of GFP3-hNup107 to the chromatin surface compared with GFP3-hNup133. See also video 4 available at Bars, 5 μm.

Videos of the four-dimensional confocal sequence shown in Fig. 6, A and B.

A single confocal section in the middle of the stack is displayed. Time is shown in were treated as for the still frames. Frame rate is 10 fps.

Nuclear Pore Complex: movie 1 | movie 2 | movie 3 | movie 4 | Cell Nucleus | PMID 11564755


<pubmed>11564755</pubmed>| J Cell Biol.


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