File:PMID11564755-Nuclear pore complexes04.mp4

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PMID11564755-Nuclear_pore_complexes04.mp4(file size: 3.92 MB, MIME type: video/mp4)

Nuclear Pore Complexes

In vivo analysis of GFP3-hNup133 and GFP3-hNup107 dynamics in mitosis. (A) Four-dimensional confocal sequence of an NRK cell stably expressing GFP3-hNup133. The sequence extends from metaphase/anaphase transition to telophase. Confocal z-stacks with six slices every 1.5 μm were acquired in 5-s intervals. A single z-slice containing the best-focused GFP signal is displayed for representative time points. Left panels show GFP fluorescence to localize the nucleoporin; right panels show the simultaneously acquired DIC images to visualize the chromosomes. Due to the low signal to noise ratio in a single confocal section, fluorescence images were contrast enhanced and smoothed with a 3 × 3 median filter to remove detector shot noise.

(B) Same as in A but from a cell stably expressing GFP3-hNup107. Time interval between z-stacks is 10 s. Note the earlier diffuse recruitment of GFP3-hNup107 to the chromatin surface compared with GFP3-hNup133. See also video 4 available at http://www.jcb.org/content/vol154/issue6. Bars, 5 μm.


Videos of the four-dimensional confocal sequence shown in Fig. 6, A and B.

A single confocal section in the middle of the stack is displayed. Time is shown in h:mm:ss.ss.Images were treated as for the still frames. Frame rate is 10 fps.



Nuclear Pore Complex: movie 1 | movie 2 | movie 3 | movie 4 | Cell Nucleus | PMID 11564755

Reference

N Belgareh, G Rabut, S W Baï, M van Overbeek, J Beaudouin, N Daigle, O V Zatsepina, F Pasteau, V Labas, M Fromont-Racine, J Ellenberg, V Doye An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. J. Cell Biol.: 2001, 154(6);1147-60 PubMed 11564755

| J Cell Biol.


Copyright

Rockefeller University Press Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

Video 4 (4.2 MB)

jcb.rupress.org/content/suppl/2001/09/10/154.6.1147.FIG6.DC1/JCB200101081.v4.mov

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