File:Mitochondria location mitosis.jpg
Figure 5 Morphological changes in the cellular mitochondrial network during mitosis. Synchronized mitochondria-tagged C9-pβGFP3′β cells were analyzed by immunofluorescence microscopy at four hours after release from the metabolic block. Typical morphologies of cells in interphase (a,f), prophase (b), metaphase (c,g), anaphase (d) and telophase (e,h) are shown at 60× magnification. The red fluorescence identifies the cytoskeletal proteins α-tubulin (a–e) and β-actin (f–h). The green fluorescence identifies mitochondria. The blue fluorescence reveals the stained nuclear DNA with the To-Pro probe.
Biogenesis and dynamics of mitochondria during the cell cycle: significance of 3'UTRs. Martínez-Diez M, Santamaría G, Ortega AD, Cuezva JM. PLoS ONE. 2006 Dec 20;1:e107.
"Nowadays, we are facing a renaissance of mitochondria in cancer biology. However, our knowledge of the basic cell biology and on the timing and mechanisms that control the biosynthesis of mitochondrial constituents during progression through the cell cycle of mammalian cells remain largely unknown. Herein, we document the in vivo changes on mitochondrial morphology and dynamics that accompany cellular mitosis, and illustrate the following key points of the biogenesis of mitochondria during progression of liver cells through the cycle: (i) the replication of nuclear and mitochondrial genomes is synchronized during cellular proliferation, (ii) the accretion of OXPHOS proteins is asynchronously regulated during proliferation being the synthesis of beta-F1-ATPase and Hsp60 carried out also at G2/M and, (iii) the biosynthesis of cardiolipin is achieved during the S phase, although full development of the mitochondrial membrane potential (DeltaPsim) is attained at G2/M. Furthermore, we demonstrate using reporter constructs that the mechanism regulating the accretion of beta-F1-ATPase during cellular proliferation is controlled at the level of mRNA translation by the 3'UTR of the transcript. The 3'UTR-driven synthesis of the protein at G2/M is essential for conferring to the daughter cells the original phenotype of the parental cell. Our findings suggest that alterations on this process may promote deregulated beta-F1-ATPase expression in human cancer."
PLoS ONE. 2006; 1(1): e107. Published online 2006 December 20. doi: 10.1371/journal.pone.0000107. Copyright Martínez-Diez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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|current||14:25, 29 March 2009||478 × 808 (63 KB)||S8600021||== Legend Title== Figure 5 Morphological changes in the cellular mitochondrial network during mitosis. Synchronized mitochondria-tagged C9-pβGFP3′β cells were analyzed by immunofluorescence microscopy at four hours after release from the metabolic blo|
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