File:Microtubule - spindle formation.mp4

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Microtubule_-_spindle_formation.mp4(file size: 386 KB, MIME type: video/mp4)

Microtubule - Spindle Formation

Time lapse movie of a porcine kidney LLCPK1 cell stably expressing EGFP-alpha tubulin. The movie shows microtubule behavior from early prophase through metaphase.

Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a MicroMax cooled CCD camera. Images were captured every 2 sec and are displayed at 7.5 frames/sec.


See also:N Rusan and P Wadsworth 2005. Centrosome fragments and microtubules are transported asymmetrically away from division plane in mitosis. J Cell Biol 168:21-28.


Reference

J Cell Biol 168:21-28.


http://www.cellimagelibrary.org/images/40269

Movie dimensions - W510xH422

Copyright

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible.

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G

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current13:31, 28 November 2017 (386 KB)Z8600021 (talk | contribs)Time lapse movie of a porcine kidney LLCPK1 cell stably expressing EGFP-alpha tubulin. The movie shows microtubule behavior from early prophase through metaphase. ==Microtubule - Spindle Formation== Cells were imaged using a Nikon Eclipse TE300 micros...
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