File:ECM elastic fibres skin.jpg

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Skin, human - elastin & van Gieson or Artery, human - elastin & eosin

Like reticular fibres, elastic fibres require special stains to be visualized. Typically elastic fibres will appear as fine, dark violet and gently undulating fibres in the tissue. Elastic fibres can form membranes - not unlike the collagen membrane in the basal lamina of epithelia. This is the case at some levels in the walls of blood vessels. Collagen and elastic fibres intermingle in the dermis, i.e. the connective tissue beneath the epithelium of the skin. Immediately beneath the epithelium both fibre types are relatively fine - they appear much thicker in the deeper parts of the dermis. At least the internal elastic lamina should be visible in the smaller arteries which course through the dermis. A combination with a second stain is necessary to visualize other tissue components. Colours visible in the sections will depend on the method used in combination with the elastin stain. Eosin gives an even pink or red colour to many tissue components. Nuclei of cells remain unstained without the inclusion of the haematoxylin in the staining solutions.

http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Connective/Connect.htm#labelastic

[[Category:Histology]

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current00:15, 27 April 2009Thumbnail for version as of 00:15, 27 April 2009400 × 500 (87 KB)S8600021 (talk | contribs)Skin, human - elastin & van Gieson or Artery, human - elastin & eosin Like reticular fibres, elastic fibres require special stains to be visualized. Typically elastic fibres will appear as fine, dark violet and gently undulating fibres in the tissue. Ela
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