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Calcium induces exocytosis of only a small fraction of lysosomes.
The NRK cell described in Fig. 4 B was treated with 10 µM calcium ionophore A23187 and imaged at five frames per second.
The movie, which is displayed at ten frames per second, shows the frames starting 115 s after calcium ionophore addition.
The boxed region contains the vesicles shown in Fig. 4 C.
Lysosomes in NRK cells were loaded with FITC dextran and cells were treated with 10 µm A23187 calcium-ionophore.
- Epifluorescence image predominantly demonstrates the perinuclear population of lysosomes.
- TIR-FM allows identification of lysosomes near the plasma membrane.
- Links: MP4 version | Movies
Jyoti K Jaiswal, Norma W Andrews, Sanford M Simon Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells. J. Cell Biol.: 2002, 159(4);625-35 PubMed 12438417
Video 3 http://jcb.rupress.org/content/suppl/2002/11/14/jcb.200208154.DC1/3
Figure 4 http://jcb.rupress.org/cgi/doifull/10.1083/jcb.200208154#FIG4
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Jaiswal JK, Andrews NW, Simon SM.
Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G