Exocytosis Movie 4

From CellBiology


<mediaplayer width='420' height='250' image="http://cellbiology.med.unsw.edu.au/cellbiology/images/1/16/Exocytosis_4-icon.jpg">File:Exocytosis 4.mp4</mediaplayer>
Exocytosis 4-icon.jpg


Calcium induces exocytosis of only a small fraction of lysosomes.

The NRK cell described in Fig. 4 B was treated with 10 µM calcium ionophore A23187 and imaged at five frames per second.

The movie, which is displayed at ten frames per second, shows the frames starting 115 s after calcium ionophore addition.

The boxed region contains the vesicles shown in Fig. 4 C.

Exocytosis 4 movie image 01.jpg

Lysosomes in NRK cells were loaded with FITC dextran and cells were treated with 10 µm A23187 calcium-ionophore.

  • Epifluorescence image predominantly demonstrates the perinuclear population of lysosomes.
  • TIR-FM allows identification of lysosomes near the plasma membrane.

Links: MP4 version | Movies


<pubmed>12438417</pubmed>| JCB

Video 3 http://jcb.rupress.org/content/suppl/2002/11/14/jcb.200208154.DC1/3

Figure 4 http://jcb.rupress.org/cgi/doifull/10.1083/jcb.200208154#FIG4

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Jaiswal JK, Andrews NW, Simon SM.

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G