Exocytosis Movie 4

From CellBiology

 

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Exocytosis

Calcium induces exocytosis of only a small fraction of lysosomes.

The NRK cell described in Fig. 4 B was treated with 10 µM calcium ionophore A23187 and imaged at five frames per second.

The movie, which is displayed at ten frames per second, shows the frames starting 115 s after calcium ionophore addition.

The boxed region contains the vesicles shown in Fig. 4 C.

Exocytosis 4 movie image 01.jpg

Lysosomes in NRK cells were loaded with FITC dextran and cells were treated with 10 µm A23187 calcium-ionophore.

  • Epifluorescence image predominantly demonstrates the perinuclear population of lysosomes.
  • TIR-FM allows identification of lysosomes near the plasma membrane.


Links: MP4 version | Movies

Reference

Jyoti K Jaiswal, Norma W Andrews, Sanford M Simon Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells. J. Cell Biol.: 2002, 159(4);625-35 PubMed 12438417

| JCB


Video 3 http://jcb.rupress.org/content/suppl/2002/11/14/jcb.200208154.DC1/3

Figure 4 http://jcb.rupress.org/cgi/doifull/10.1083/jcb.200208154#FIG4


Rockefeller University Press Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

Jaiswal JK, Andrews NW, Simon SM.



Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G