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View of many Carriers
Movie shows exocytotic vesicles being transported from the Golgi apparatus to the cell periphery. The sequence, taken at 30 frames/s, shows a part of the cell surface imaged in TIR-FM. The cell edge is in the upper left, the Golgi is in the lower right corner (original movie pixel size, 256 nm).
As can be seen on the extreme left and right, fusion occurs with a bright local burst of fluorescence followed by a spread of the fluorescence.
The carrier on the extreme left was observed doing transport, docking, and fusion during the acquisition time.
The two carriers on the extreme right did not move significantly before they fused.
|| There are three types of behaviors of the post-Golgi carriers:
- carriers that are stationary
- carriers that are moving, but not fusing;
- carriers that fuse to the plasma membrane.
- Links: 1.3 Mb MP4 version | 3.2 Mb MP4 version | Movies
J Schmoranzer, M Goulian, D Axelrod, S M Simon Imaging constitutive exocytosis with total internal reflection fluorescence microscopy. J. Cell Biol.: 2000, 149(1);23-32 PubMed 10747084
Video 1 http://jcb.rupress.org/content/suppl/2000/06/07/149.1.23.F2.DC1 video was increase in size.
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Schmoranzer J, Goulian M, Axelrod D, Simon SM.
Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G