Exocytosis Movie 3

From CellBiology

 

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Post-Golgi carriers-icon.jpg

View of many Carriers

Movie shows exocytotic vesicles being transported from the Golgi apparatus to the cell periphery. The sequence, taken at 30 frames/s, shows a part of the cell surface imaged in TIR-FM. The cell edge is in the upper left, the Golgi is in the lower right corner (original movie pixel size, 256 nm).

As can be seen on the extreme left and right, fusion occurs with a bright local burst of fluorescence followed by a spread of the fluorescence.

The carrier on the extreme left was observed doing transport, docking, and fusion during the acquisition time.

The two carriers on the extreme right did not move significantly before they fused.

Post-Golgi carriers 01.jpg There are three types of behaviors of the post-Golgi carriers:
  1. carriers that are stationary
  2. carriers that are moving, but not fusing;
  3. carriers that fuse to the plasma membrane.


Links: 1.3 Mb MP4 version | 3.2 Mb MP4 version | Movies

Reference

J Schmoranzer, M Goulian, D Axelrod, S M Simon Imaging constitutive exocytosis with total internal reflection fluorescence microscopy. J. Cell Biol.: 2000, 149(1);23-32 PubMed 10747084

| JCB


Video 1 http://jcb.rupress.org/content/suppl/2000/06/07/149.1.23.F2.DC1 video was increase in size.



Rockefeller University Press Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

Schmoranzer J, Goulian M, Axelrod D, Simon SM.



Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G