Exocytosis Movie 1

From CellBiology

 

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Exocytic transport intermediates carrying GPI-GFP

GPI-GFP was expressed in COS-7 cells. All fluorescence outside of the region indicated in the prebleach image was eliminated by photobleaching.


Note that the fluorescence intensity of the Golgi region is saturated (pixel value equals 255 for 8-bit images) in all of these images to allow visualization of the less bright transport intermediates.

Times in subsequent panels are relative to the end of the photobleach. Bar, 5 μm.


Links: MP4 version | Exocytosis Movie 2 | Movies

Reference

B J Nichols, A K Kenworthy, R S Polishchuk, R Lodge, T H Roberts, K Hirschberg, R D Phair, J Lippincott-Schwartz Rapid cycling of lipid raft markers between the cell surface and Golgi complex. J. Cell Biol.: 2001, 153(3);529-41 PubMed 11331304

| PMC2190578 | JCB


Copyright

Rockefeller University Press Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

Video 3B (9.7MB) See Fig. 3 Original file: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190578/bin/jcellbiol_153_3_529_F3v2_index.html

movie increased in size.

Supplemental video available at http://www.jcb.org/cgi/content/full/153/3/529/DC1.

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G