Endosome Movie 1

From CellBiology


SBD is a non-toxic probe consisting of a 25-amino acid sphingolipid binding domain, the SBD, derived from the amyloid peptide Abeta, and conjugated by a neutral linker with an organic fluorophore. SBD was the name given to a motif originally recognized by Fantini et al in a number of glycolipid-associated proteins, and was proposed to interact with sphingolipids in membrane microdomains.

Movie showing SBD (green) incorporation into motile endolysosomal compartments of SH-SY5Y neuroblastomas labeled with Lysotracker (red).

Movie was taken by widefield fluorescence microscopy, and spans 5.7 min of real time, 30 min after SBD labelling (2 µM, 15 min, 37°C), at 1.2 sec/ frame, and assembled at 30 fps. (4.41 MB MOV)

Links: MP4 version | Quicktime version | Movies


<pubmed>18716682</pubmed>| PMC2518528 | PLoS One.


Copyright: © 2008 Steinert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Steinert S, Lee E, Tresset G, Zhang D, Hortsch R, et al. (2008) A Fluorescent Glycolipid-Binding Peptide Probe Traces Cholesterol Dependent Microdomain-Derived Trafficking Pathways. PLoS ONE 3(8): e2933. doi:10.1371/journal.pone.0002933

Original File name: Journal.pone.0002933.s004.mov


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