Endosome Movie 1

From CellBiology

 

SBD is a non-toxic probe consisting of a 25-amino acid sphingolipid binding domain, the SBD, derived from the amyloid peptide Abeta, and conjugated by a neutral linker with an organic fluorophore. SBD was the name given to a motif originally recognized by Fantini et al in a number of glycolipid-associated proteins, and was proposed to interact with sphingolipids in membrane microdomains.


Movie showing SBD (green) incorporation into motile endolysosomal compartments of SH-SY5Y neuroblastomas labeled with Lysotracker (red).


Movie was taken by widefield fluorescence microscopy, and spans 5.7 min of real time, 30 min after SBD labelling (2 µM, 15 min, 37°C), at 1.2 sec/ frame, and assembled at 30 fps. (4.41 MB MOV)



Links: MP4 version | Quicktime version | Movies


Reference

Steffen Steinert, Esther Lee, Guillaume Tresset, Dawei Zhang, Ralf Hortsch, Richard Wetzel, Sarita Hebbar, Jeyapriya Raja Sundram, Sashi Kesavapany, Elke Boschke, Rachel Kraut A fluorescent glycolipid-binding peptide probe traces cholesterol dependent microdomain-derived trafficking pathways. PLoS ONE: 2008, 3(8);e2933 PubMed 18716682

| PMC2518528 | PLoS One.


Copyright

Copyright: © 2008 Steinert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Citation: Steinert S, Lee E, Tresset G, Zhang D, Hortsch R, et al. (2008) A Fluorescent Glycolipid-Binding Peptide Probe Traces Cholesterol Dependent Microdomain-Derived Trafficking Pathways. PLoS ONE 3(8): e2933. doi:10.1371/journal.pone.0002933

Original File name: Journal.pone.0002933.s004.mov

http://www.plosone.org/article/fetchSingleRepresentation.action?uri=info:doi/10.1371/journal.pone.0002933.s004

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G