Endocytosis Movie 1

From CellBiology

 

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Endocytosis

Movie shows the endocytic transport to the Golgi complex.


Movie shows the endocytic transport intermediates delivering STxB, CTxB, and GPI-GFP to the Golgi complex.

Selective photobleaching and time-lapse imaging to visualize delivery of GPI-GFP to the Golgi complex. A series of images of a small region of the cell adjacent to the Golgi complex were rapidly collected immediately after photobleaching of the GPI-GFP fluorescence associated with the Golgi complex and the surrounding area (Video 4, A–D). Small transport intermediates containing GPI-GFP were observed tracking into the photobleached Golgi region where they disappeared, presumably due to fusion with the Golgi complex and subsequent dispersal of fluorescence.

Links: 1.5 Mb MP4 version | 4 Mb MP4 version | Movies
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Reference

B J Nichols, A K Kenworthy, R S Polishchuk, R Lodge, T H Roberts, K Hirschberg, R D Phair, J Lippincott-Schwartz Rapid cycling of lipid raft markers between the cell surface and Golgi complex. J. Cell Biol.: 2001, 153(3);529-41 PubMed 11331304

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Video 4A http://jcb.rupress.org/content/suppl/2001/04/30/153.3.529.F5.DC1


Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G