Cells - RGC5

From CellBiology

Introduction

Originally characterised as rat[1]. A more recent paper suggests that this is actually a mouse cell line[2] and not retinal ganglion in origin.


This retinal cell line was generated and supplied by Associate Professor Neeraj Agarwal, Associate Professor of Cell Biology & Genetics, UNT Health Science Center, Fort Worth, TX 76107, nagarwal@hsc.unt.edu. Prof Agarwal should be cited and contacted directly with all requests concerning this cell line.

The information below is for educational purposes only.


Cell Culture: 3T3 | B35 | C2C12 | C17-2 | HT4 | IGR3 | NT2 | PC12 | RGC5

Growth

Undifferentiated

Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin incubated at 37°C in 5% CO2.

Differentiation

RGC-5 cells then differentiated the next day by replacing culture media with staurosporine (316 nM).

Properties

RGC5

Retinal Ganglion Cell line 5

Positive

Function

Thy-1

Membrane-

CNTF

Ciliary NeuroTrophic Factor

Secreted- growth factor

GDNF

Glia Derived Neurotrophic Factor

Secreted- growth factor

NT3

NeuroTrophin 3

Secreted- growth factor

NT4

NeuroTrophin 4

Secreted- growth factor

TrkA

Membrane- Growth Factor receptor

TrkB

Membrane- Growth Factor receptor

TrkC

Membrane- Growth Factor receptor

Brn3C

Neuritin

NF-160

Neurofilament protein Mr160kD

Neuron Cytoskeleton- intermediate filament protein

NF200

Neurofilament protein Mr200kD

Neuron Cytoskeleton- intermediate filament protein

Negative

Function

GFAP

Glia Cytoskeleton- intermediate filament protein

Syntoxin
(8A-1)

Amacrine Cell marker

HPC-1

Horizontal Cell marker

References

  1. <pubmed>11165366</pubmed>
  2. <pubmed>19443730</pubmed>


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