Cells - HT4

From CellBiology

Introduction

Temperature sensitive neural cell line expressing SV40 small T at lower temperature, inhibited at high temperature and therefore ceases proliferation and differentiates.

Culture Notes - Cells originally from J Bamburg.

Cell Culture: 3T3 | B35 | C2C12 | C17-2 | HT4 | IGR3 | NT2 | PC12 | RGC5


Growth Media

DMEMF10 Tissue: embryonal carcinoma, malignant; testis; from metastatic site:lung

Growth Properties: monolayer

  • Undifferentiated - 33°C in Dulbecco’s modified Earl’s medium (DMEM) containing 10% FCS, 100 U/ml penicillin, 100 pg/ml streptomycin, and 500 pg/ml G418.
  • Differentiated - 39°C in N5 media.

References


<pubmed>2033646 </pubmed>


Links

http://www.invitrogen.com/site/us/en/home/support/Cell-Lines-Browse/Cell-Lines-Results/Cell-Lines-Detail.307.html