Cells - HT4
Temperature sensitive neural cell line expressing SV40 small T at lower temperature, inhibited at high temperature and therefore ceases proliferation and differentiates.
Culture Notes - Cells originally from J Bamburg.
DMEMF10 Tissue: embryonal carcinoma, malignant; testis; from metastatic site:lung
Growth Properties: monolayer
- Undifferentiated - 33°C in Dulbecco’s modified Earl’s medium (DMEM) containing 10% FCS, 100 U/ml penicillin, 100 pg/ml streptomycin, and 500 pg/ml G418.
- Differentiated - 39°C in N5 media.