Cells - C2C12
This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel.The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture. Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum. Tested and found negative for ectromelia virus (mousepox).
ATCC Number: CRL-1772 Organism: Mus musculus (mouse) ATCC Number: CRL-1772
Organism: Mus musculus (mouse)
Tissue: muscle; myoblast
Depositors: B. Paterson
FluidRenewal: twice weekly
SubCulturing: Remove medium, rinse cell sheet with 0.25% trypsin, and add 1 to 2ml of fresh trypsin. Let the flask sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.
SplitRatio: A ratio of 1:3 to 1:6 is recommended
Growth Properties: monolayer
Name: C2C12 ECACC Ref No: 91031101
Comments: Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.