Cells - C17-2

From CellBiology

Introduction

Multipotent neural cell lines were generated via retrovirus-mediated v-myc transfer into murine cerebellar progenitor cells.

Cell Culture: 3T3 | B35 | C2C12 | C17-2 | HT4 | IGR3 | NT2 | PC12 | RGC5


Growth Media

DMEMF10 Tissue:neuron

Morphology: undifferentiated cells appear epithelial with short extensions. FluidRenewal: twice weekly

SubCulturing: Remove medium, rinse cell sheet with 0.25% trypsin, and add 1 to 2ml of fresh trypsin. Let the flask sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

SplitRatio: A ratio of 1:3 to 1:6 is recommended

Growth Properties: monolayer

Comments:

References:

Snyder EY, et al. [See Related Articles] Multipotent neural cell lines can engraft and participate in development of mouse cerebellum.Cell 1992 Jan 10;68(1):33-51 Kitchens DL, et al. [See Related Articles] FGF and EGF are mitogens for immortalized neural progenitors. J Neurobiol. 1994 Jul;25(7):797-807. PMID: 8089657; UI: 94376081. Snyder EY, et al. [See Related Articles] Multipotent neural precursors can differentiate toward replacement of neurons undergoing targeted apoptotic degeneration in adult mouse neocortex. Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11663-8. Lynch WP, et al. [See Related Articles] Neural stem cells as engraftable packaging lines can mediate gene delivery to microglia: evidence from studying retroviral env-related neurodegeneration. J Virol. 1999 Aug;73(8):6841-51. Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% also with Pen/strep 1%.


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