Cells - 3T3

From CellBiology

Introduction

The 3T3 cell line is a fibroblast cell culture established from disaggregated tissue of an embryonic albino Swiss mouse (Mus musculus).

The original 3T3 line was established in the 1960s by George Todaro and Howard Green.

These cells were used to show the difference between cell mortality and a cell's ability to undergo oncogenic transformation (3T3 cells could grow indefinitely and did not instigate tumor growth).

Cell Culture: 3T3 | B35 | C2C12 | C17-2 | HT4 | IGR3 | NT2 | PC12 | RGC5

ATCC

Name: NIH 3T3 ECACC Ref No: 93061524

Comments: Established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.

Morphology: Fibroblast Description Key Words: Mouse Swiss NIH embryo contact inhibited

Tissue: embryo Species: Mouse

Medium: DMEM + 2mM Glutamine + 10% Calf Serum (CS).

Sub Culture Routine: Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of foetal calf serum is not recommended. R.C.D: No

Karyotype: Not Specified Passage Number: 136

Receptors: Histocompatability:

Products: Reference: J Virol 1969;4:549; Cell 1979;16:63; Cell 1979;16:347

Jainchill JL, et al. [See Related Articles] Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J Virol. 1969 Nov;4(5):549-53. No abstract available. Andersson P, et al. [See Related Articles] A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell. 1979 Jan;16(1):63-75. Copeland NG, et al. [See Related Articles] Transfection by exogenous and endogenous murine retrovirus DNAs. Cell. 1979 Feb;16(2):347-56. No abstract available.