Bacteria Cell Division Movie 1
| This movie shows the process of bacterial cell division. Note the cells grow in size and then divide, this process is called "binary fission".
The lefthand image is bright field microscopy the righthand image is fluorescence microscopy of the same cells.
Transient SodA:mcherry expression among cell division.
Left: Sequence of images showing an overlay of phase contrast and YFP fluorescence of a colony from a cell that carry a foci of the protein aggregation marker ibpA-YFP;
Right: Sequence of images showing an overlay of mcherry fluorescence and cell contours as retrieved by the annotation software. Cell contour of cell containing identified foci of the protein aggregation marker ibpA-YFP (magenta) are in yellow and contour of other cells are in green.
Each frame was taking every 5 min.
Ducret A, Maisonneuve E, Notareschi P, Grossi A, Mignot T, et al. (2009) A Microscope Automated Fluidic System to Study Bacterial Processes in Real Time. PLoS ONE 4(9): e7282. doi:10.1371/journal.pone.0007282
Editor: Christophe Herman, Baylor College of Medicine, United States of America
Received: July 31, 2009; Accepted: September 8, 2009; Published: September 30, 2009
© 2009 Ducret et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Original file name: Journal.pone.0007282.s002.mov