2016 Lab 12

From CellBiology

Microarray Laboratory

Microarray Slide

Introduction

Please meet me first at the new Wallace Wurth teaching lab (W06).

  1. Cover any additional revision issues before the theory exam.
  2. Complete the course CATEI. This will be an online form requiring you to log on to MyUNSW.

The second half of this laboratory will be a visit to the UNSW The Ramaciotti Centre for Gene Function Analysis.

  • Enclosed footwear will be required.
  • You will have the opportunity to see the practical aspects of the new methods of cell analysis.
  • Have a look through the centre's website before you visit and the other links to information on microarrays.





Dr Helen Speirs

The Ramaciotti Centre Level 1, room 131 or 137 Biological Sciences Building (D26)

After the facility tour the practical class has finished.

Links

Microarray technology

References

Textbooks

Essential Cell Biology

  • Essential Cell Biology Chapter

Molecular Biology of the Cell

Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter New York and London: Garland Science; c2002

Molecular Cell Biology

Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James E. New York: W. H. Freeman & Co.; c1999

Madame Curie Bioscience Database

Chapters taken from the Madame Curie Bioscience Database (formerly, Eurekah Bioscience Database) Eurekah.com and Landes Bioscience and Springer Science+Business Media; c2009

Search Online Textbooks

Books

PubMed

  • PubMed is a service of the U.S. National Library of Medicine that includes over 18 million citations from MEDLINE and other life science journals for biomedical articles back to 1948. PubMed includes links to full text articles and other related resources. PubMed
  • PubMed Central (PMC) is a free digital archive of biomedical and life sciences journal literature at the U.S. National Institutes of Health (NIH) in the National Library of Medicine (NLM) allowing all users free access to the material in PubMed Central. PMC
  • Online Mendelian Inheritance in Man (OMIM) is a comprehensive compendium of human genes and genetic phenotypes. The full-text, referenced overviews in OMIM contain information on all known mendelian disorders and over 12,000 genes. OMIM
  • Entrez is the integrated, text-based search and retrieval system used at NCBI for the major databases, including PubMed, Nucleotide and Protein Sequences, Protein Structures, Complete Genomes, Taxonomy, and others Entrez

Search Pubmed


Reviews

Yiping Shen, Bai-Lin Wu Microarray-based genomic DNA profiling technologies in clinical molecular diagnostics. Clin. Chem.: 2009, 55(4);659-69 PubMed 19233918

Heiko Andresen, Frank F Bier Peptide microarrays for serum antibody diagnostics. Methods Mol. Biol.: 2009, 509;123-34 PubMed 19212718

S E Wildsmith, F J Elcock Microarrays under the microscope. MP, Mol. Pathol.: 2001, 54(1);8-16 PubMed 11212888

M Q Zhang Large-scale gene expression data analysis: a new challenge to computational biologists. Genome Res.: 1999, 9(8);681-8 PubMed 10447504

M Schena, R A Heller, T P Theriault, K Konrad, E Lachenmeier, R W Davis Microarrays: biotechnology's discovery platform for functional genomics. Trends Biotechnol.: 1998, 16(7);301-6 PubMed 9675914


2016 Course Content

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2016 Revision


Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Cell Knockout Methods | Cytoskeleton Exercise | Immunochemistry | Project Work | Confocal Microscopy | Tissue Culture | Stem Cells Lab | Microarray Visit


2016 Projects: Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G