2015 Lab 11

From CellBiology

Stem Cells

As part of the assessment for this course, you will give a 15 minutes journal club presentation in Lab 11 (28 May 4-6 pm). Each Group will discuss a recent original research article (not a review!) on stem cell biology or technology.

  • During the presentation it works best if one student discusses the introduction, the second the results section, and the third the discussion section.
  • Please note that one slide takes about 1 minute to talk through.
  • So do not use more than 15 to 20 slides total.
  • Please read through attached document for tips for presentations.

You will receive a group mark based on presentation content, insight and comprehension, and presentation and slide style.

List of Papers and Student Hints PDF

Stem Cell Papers

1 Marc van de Wetering, Hayley E Francies, Joshua M Francis, Gergana Bounova, Francesco Iorio, Apollo Pronk, Winan van Houdt, Joost van Gorp, Amaro Taylor-Weiner, Lennart Kester, Anne McLaren-Douglas, Joyce Blokker, Sridevi Jaksani, Sina Bartfeld, Richard Volckman, Peter van Sluis, Vivian S W Li, Sara Seepo, Chandra Sekhar Pedamallu, Kristian Cibulskis, Scott L Carter, Aaron McKenna, Michael S Lawrence, Lee Lichtenstein, Chip Stewart, Jan Koster, Rogier Versteeg, Alexander van Oudenaarden, Julio Saez-Rodriguez, Robert G J Vries, Gad Getz, Lodewyk Wessels, Michael R Stratton, Ultan McDermott, Matthew Meyerson, Mathew J Garnett, Hans Clevers Prospective derivation of a living organoid biobank of colorectal cancer patients. Cell: 2015, 161(4);933-45 PubMed 25957691

| Cell

2 M Takasato, P X Er, M Becroft, J M Vanslambrouck, E G Stanley, A G Elefanty, M H Little Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. Nat. Cell Biol.: 2014, 16(1);118-26 PubMed 24335651

| Nat Cell Biol.

3 Hiroyuki Kamao, Michiko Mandai, Satoshi Okamoto, Noriko Sakai, Akiko Suga, Sunao Sugita, Junichi Kiryu, Masayo Takahashi Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application. Stem Cell Reports: 2014, 2(2);205-18 PubMed 24527394

| Stem Cell Reports.

4 Won Kyung Song, Kyung-Mi Park, Hyun-Ju Kim, Jae Ho Lee, Jinjung Choi, So Young Chong, Sung Han Shim, Lucian V Del Priore, Robert Lanza Treatment of macular degeneration using embryonic stem cell-derived retinal pigment epithelium: preliminary results in Asian patients. Stem Cell Reports: 2015, 4(5);860-72 PubMed 25937371

| Stem Cell Reports.

5 Yuxuan Wu, Dan Liang, Yinghua Wang, Meizhu Bai, Wei Tang, Shiming Bao, Zhiqiang Yan, Dangsheng Li, Jinsong Li Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell: 2013, 13(6);659-62 PubMed 24315440

| Cell Stem Cell.

6 Chengzu Long, John R McAnally, John M Shelton, Alex A Mireault, Rhonda Bassel-Duby, Eric N Olson Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Science: 2014, 345(6201);1184-8 PubMed 25123483

| Science.

Paper Group

Group 1

--Z3330686 (talk) 16:23, 14 May 2015 (EST)

--Z5050795 (talk) 16:24, 14 May 2015 (EST)

--Z5016650 (talk) 21:06, 14 May 2015 (EST)

--Z5050801 (talk) 17:00, 21 May 2015 (EST)

--Z5050826 (talk) 17:01, 21 May 2015 (EST)

Group 2

--Z5049401 (talk) 16:17, 14 May 2015 (EST)

--Z3418779 (talk) 16:38, 14 May 2015 (EST)

--Z3415735 (talk) 18:59, 15 May 2015 (EST)

--Z3374116 (talk) 16:50, 21 May 2015 (EST)

--Z3461763 (talk) 17:21, 21 May 2015 (EST)

Group 3

--Z3375627 (talk) 16:26, 14 May 2015 (EST)

--Z3417843 (talk) 16:27, 14 May 2015 (EST)

--Z3333429 (talk) 16:31, 14 May 2015 (EST)

--Z3462921 (talk) 16:37, 14 May 2015 (EST)

Group 4

--Z5050822 (talk) 16:17, 14 May 2015 (EST)

--Z3415779 (talk) 16:18, 14 May 2015 (EST)

--Z5030452 (talk) 16:18, 14 May 2015 (EST)

--Z5050800 (talk) 16:23, 14 May 2015 (EST)

Group 5

--Z3332339 (talk) 16:20, 14 May 2015 (EST)

--Z5049618 (talk) 16:20, 14 May 2015 (EST)

--Z3417458 (talk) 16:22, 14 May 2015 (EST)

--Z3463637 (talk) 16:24, 14 May 2015 (EST)

--Z3462211 (talk) 16:09, 21 May 2015 (EST)

Group 6

--Z3459996 (talk) 16:20, 14 May 2015 (EST)

--Z3459282 (talk) 11:00, 16 May 2015 (EST)

--Z3466260 (talk) 16:45, 21 May 2015 (EST)

--Z3459592 (talk) 16:46, 21 May 2015 (EST)

Presentation Hints for Students

  • Do not use cheat sheets and do not learn your presentation literally by heart. Make sure that you know and understand what you want to get across. Explain carefully. Use your slides as cheat sheets. Make eye contact with your audience and get a feel for whether they understand your story.
  • Keep your presentation short and concise. Not every detail of the article needs to be discussed in the presentation, but limit it to the bare minimum that is required to get the main message of the article across. For instance, do not go into too much detail in method sections. Not all nitty-gritty detail of the results needs to be discussed. The less info your audience has to take in, the higher the chance that they will understand your story.
  • Don’t just put your slides up while you are presenting, but talk your audience carefully through them. Slides are an indispensable part of the presentation. Each item on your slides should be relevant and addressed and highlighted with pointer, fingers, stick. Slide shows are indispensable for a presentation, as is the presenter. They should support and enhance a presentation, they should aid your audience in understanding.
  • Talk your audience through each of the figures on your slides. Figures may be obvious to you, but not to your audience unless you explain them carefully. So explain what experiment has been carried out, and what is displayed in the figure:
    • on the X and Y-axes
    • what the bars represent in diagrams
    • the tissues/cell types displayed
    • the bands on Western blot, RNA and DNA gels,
    • What colors represent colors in immunostainings, etc etc.
  • Please note that you only need to highlight this experimental detail that is necessary to get the main message of the figure across.
  • Conclude a (results) slide with a concluding/summarizing remark that should cover the main message of this particular slide.
  • Annotate the figures in your presentation carefully but sparingly. Label panels, axes, images etc so that figures are self-explicatory.
  • Do not use too much text on your slides.
  • To stay in control the presenter should flick through the slide show. Not another member of the team.
  • If you didn’t understand the articles in depth, read a recent review or even go back to text books to acquire the basic knowledge. Also, if you discuss results of a crucial experiment but do not understand the technology. Please go back to the original references or your text books to read up on this technology. You should be on top of everything you say or write up in your slides.
  • Stick to your time. Don’t make too many slides. Each slide should take about a minute on average to talk through. Try to avoid acronyms and abbreviation.

2015 Course Content

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2015 Revision

Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Cell Knockout Methods | Cytoskeleton Exercise | Immunochemistry | Project Work | Confocal Microscopy | Tissue Culture | Stem Cells Lab | Microarray Visit

2015 Projects: Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G