2014 Lab 9

From CellBiology

Introduction

As I announced at Tuesday lecture, due to a double booking this morning I will be giving the second lecture, Cell Death Lecture 2 in the first hour of this lab.


Lab 9 Assessment

You are a research scientist who has isolated cells that are growing in tissue culture. These normal cells when in their normal organ/tissue die after 2 weeks and are replaced as part of that organ/tissue's normal function.


With your group design an experiment(s) that would identify how (the mechanism) these cell would normally die.

  1. Write a hypothesis that you are going to test.
  2. Write aims of your experiment.
  3. Identify key techniques and procedures used in your investigation (Spell these out in some detail).
  4. Identify suppliers that have resources that you will need for your study (create links to the supplier resource pages, kits, antibodies etc).
  5. Now prepare a flow diagram of how the experiment will be carried out and analysed.
  6. What will different experimental results (outcomes) mean.



2014 Course Content

Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cell Mitochondria | Cell Junctions | Cytoskeleton Introduction | Cytoskeleton - Intermediate Filaments | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | 2013 Revision | Development | 2014 Revision


Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Immunochemistry | Cell Knockout Methods | Cytoskeleton Exercise | Confocal Microscopy | Tissue Culture | Microarray Visit | Stem Cells Lab

2014 Projects: Group 1 | Group 2 | Group 3 | Group 4

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G