2010 Foundations Histology Expert Tutorial
- Wed 21 March 2010 12:00 PM - 1:00 PM SGR4 Mark Hill
About Expert Tutorials
- Ask your SGS for specific topics or questions you would like to ask the Expert Tutor.
- Prioritize the questions.
- Submit them by email well before the expert tutorial, as they can them be reviewed by the expert to look for common problems in understanding.
- Read what the class/lecture is designed to do and the key concepts.
- Have all the issues been met by the content provided.
- Make sure you have something to report back.
About This Expert Tutorial
- Don't get too carried away with details remember these are supposed to be introductory.
- You will cover many of these topics again in other settings during your course.
UNSW Virtual Slides
- Foundations http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/A58844
- Introduction to microscopy http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/E58365
- Introduction to Histology: Cells and Tissues http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/1D40FC
- Department of Pathology, University of Iowa http://www.path.uiowa.edu/virtualslidebox/
Science Practical - Introduction to Microscopy
- Not much to cover from this practical as it was an introduction.
- Generally we cannot microscopically see tissues unless they have been stained with coloured dyes and labels that bind specifically to cellular and extracellular components.
- You do not need to know the all the histological stains used, though be aware that hematoxylin and eosin stain (H&E stain), are a quick and good contrast of tissue features.
- basic dye hematoxylin - colours basophilic structures blue-purple (DNA, RNA)
- acidic eosin - colours eosinophilic structures pink-red (protein)
- Some other stains, for reference only Histology Stains
- Virtual Microscope - Introduction to Microscopy
Science Practical - Introduction to histology - Cells and Tissue
This laboratory class is designed to:
- Obtain an understanding of the histological appearance of the basic tissues namely epithelium, connective tissue, muscle and nervous tissue.
- To examine unique cellular characteristics of each of the basic tissues.
- To correlate the functions of the basic tissues with their morphology.
Key concepts addressed:
- A histological examination of selected examples of each of the basic tissues will be addressed.
- Identify the various types of tissue characteristics and relate these to their function.
- Examine cellular morphology for each of the basic tissues.
Reference: Junqueira and Carneiro (2005). Basic Histology, 11th Edition, General reading of Chapters 4, 5, 6, 7, 8, 9 and 10 to obtain an overview of the basic tissues and the variety of cell types involved. NB: This is available online. However students are expected to purchase the latest edition as one of the required textbooks. Access via Lange Educational Library from Sirius. http://sirius.library.unsw.edu.au
- Basic background to the types of tissues and their organization in the body. Look at examples of these within the lab.
- Broadly Epithelia or Connective tissue cellular organization
- most tissues and organs contain both types (for example, skin has an epithelium epidermis and a connective tissue dermis and hypodermis) Integumentary
- classified by: number of cell layers (simple, pseudostratified, stratified), cell shape (columnar, cuboidal, squamous) and any specializations.
- Have a specialized extracellular matrix (ECM) on which basal cells sit, the basal lamina, and generally little or none ECM between the cells.
- as surfaces or protective boundaries between environments and tissues, secretion or absorption.
- consists of cells separated by varying amounts of extracellular matrix (ECM).
- classified by ECM components (eg reticular fibres), organization (eg loose connective tissue and dense connective tissues) and cell types (eg adipose)
- blood is a liquid connective tissue
- many different functions, support, strength, tissue integrity, connectivity, etc.