This laboratory is an introduction to Immunological methods for analysis of cells and tissues in cell biology. The laboratory handout and textbook alone contain enough
information as an introduction to the subject for this level of study. If
you are interested in further reading, I have also included below links to
more detailed textbooks and websites with further information and images. Please note this
additional information is not necessarily examinable, but may be useful if
you have not previously studied biology.
Understand historic background of immunology
Brief understanding of immune response
Brief understanding of Polyclonal antibodies
Brief understanding of Monoclonal antibodies
Understand major antibody techniques
Page Links: Start Here | NLM Online Textbooks | History | Immune Response | Polyclonal Antibodies | Monoclonal Antibodies | Techniques |References | Related Topics | Commercial Resources
Immunology is an entire subject to itself, so this lab is only an introduction to the key concepts you will require to understand the practical side of its application to cell biology. There are also a huge number of different techniques that use the core of immunology (the interaction/binding between an antigen and and antibody).
NCBI
MBoC | Publisher (Garland) MBoC
NCBI
MCB | Publisher
(Freeman) MCB
Growth of Animal Cells in Culture
Search MBC - "
Cell Culture"
NCBI The Cell | Publisher
(Sinauer) The Cell
Growth of Animal Cells in Culture
Search The Cell - "Cell Culture"
Oklahoma State University Department of Biochemistry and Molecular Biology
OSU Biochemistry and Molecular Genetics of Antibodies
Commercial Resources
Chemicon - Introduction to Antibodies
A downloadable online Introduction to Antibodies PDF
(27 pages, 2.5 Mb) introducing antibodies and techniques.
Journal of Histochemistry and Cytochemistry
Histochemistry and Cell Biology
Biotechnic and Histochemistry
European Journal of Histochemistry
Journal of Molecular Histology
organelle labels, aldehyde cross-linking, artefatcs of fixation, immunochemistry, monoclonal antibodies
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Lab01 Background
Lab02 Introduction to Tissue Culture
Lab03 Introduction to Fixation
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NCBI Bookshelf
This Lab is an introduction to antibodies. As such, I expect at this stage only a broad understanding of concepts, that will be built upon in later Labs.
Please email Dr Mark Hill if you wish to make a comment about this current project.