This laboratory is an introduction to methods of cell fractionation for analysis of cells and tissues in cell biology. The laboratory handout and textbook alone contain enough
information as an introduction to the subject for this level of study. If
you are interested in further reading, I have also included below links to
more detailed textbooks and websites with further information and images. Please note this
additional information is not necessarily examinable, but may be useful if
you have not previously studied biology.
Understand historic background of cell fractionation
Understand development of major cell fractionation techniques
Brief understanding of cell fractionation
Brief understanding of protein chemistry separation
This Lab content is covered in some good detail in Molecular Biology of the Cell (III. Methods, 8. Manipulating Proteins, DNA, and RNA) Fractionation of Cells We will be using this section as a guide for the Laboratory.
Nuclei Diameter (5-10 µm) Density (1.4 g/cm3)
Mitochondria Diameter (1-2 µm) Density (1.1 g/cm3)
Ribosomes Diameter (0.02 µm) Density (1.6 g/cm3)
Lysosomes Diameter (1-2 µm) Density (1.1 g/cm3)
Rough Endoplasmic Reticulum Density (1.2 g/cm3) due to bound ribosomes
Golgi vesicles Density (1.14 g/cm3)
Plasma Membranes Density (1.12 g/cm3)
Source: Carolina Tips, Nov. 1, 1973, and Mol. Cell Biol. 1999.