This laboratory is an introduction to tissue or cell culture. The laboratory handout and textbook alone contain enough
information as an introduction to the subject for this level of study. If
you are interested in further reading, I have also included below links to
more detailed textbooks and websites with further information and images. Please note this
additional information is not necessarily examinable, but may be useful if
you have not previously studied biology.
Understand historic background of cell culture
Understand growth of animal cells in culture
Brief understanding of nutritional requirements of cells
Understand the difference between a primary cell culture and cell line culture
Brief understanding of how cell lines are established
Page Links: Start Here | NLM Online Textbooks | Web Links | Search | References | Related Topics | Commercial Resources
Lets start by quick visit to the Tissue Culture Lab where in 6 quick pages we can see the major pieces of equipment in the lab and then look at the CBL Methods page on Tissue Culture.
Then trace the textbook summary of historic landmarks in the development of cell culture, which began about a century ago.
Then look at textbook links below for more details and finally the external links to Cell Banks and some other labs and methods.
Remember that this is an introduction to cell culture and we will come back and revisit the topic in several classes.
Lab 2 Powerpoint Tissue Culture Slides.
NCBI
MBoC | Publisher (Garland) MBoC
Table 8-3. Some Landmarks in the Development of Tissue and Cell Culture
Composition of a Typical Medium Suitable for the Cultivation of Mammalian Cells
Serum-free, Chemically Defined Media Permit Identification of Specific Growth Factors
Serum-free, Chemically Defined Media Permit Identification of Specific Growth Factors
Some Commonly Used Cell Lines
Search MBoC4 - "
Cell Culture"
NCBI
MCB | Publisher
(Freeman) MCB
Growth of Animal Cells in Culture
Figure 6-5. Stages in the establishment of a cell culture
Search MBC - "
Cell Culture"
NCBI The Cell | Publisher
(Sinauer) The Cell
Growth of Animal Cells in Culture
Key Experiment : Animal Cell Culture
Search The Cell - "Cell Culture"
Cell Banks American Type Culture Collection (ATCC) | European Collection of Cell Cultures (ECACC)
Other Cell Culture Pages Julie B. Wolf (UMBC)Tissue Culture Methods-1
Commercial Resources
Corning :
cell culture |
Personal Safety in the Laboratory
cryogenic storage
Culture of Animal Cells: A Manual of Basic Technique (3rd edition) Freshney, R. I., 1994., Wiley-Liss, New York.
Biohazard Hood to Prevent Infection During Microbiological Procedures Lewis L. Coriell and Gerard J. Mcgarrity Appl Microbiol. 1968 December; 16(12): 1895-1900.
Historic article on biohazard hood design
Safe biotechnology (5). Recommendations for safe work with animal and human cell cultures concerning potential human pathogens. Frommer W, Archer L, Boon B, Brunius G, Collins CH, Crooy P, Doblhoff-Dier O, Donikian R, Economidis J, Frontali C, et al.
Appl Microbiol Biotechnol. 1993 May;39(2):141-7.
Cell culture models of differentiation. Watt FM., FASEB J. 1991 Mar 1;5(3):287-94. Review.
read the article
The golden age of retinal cell culture. Seigel GM., Mol Vis. 1999 Apr 19;5:4. Review.
read the article
Comparing the properties of neuronal culture systems: a shopping guide for the cell biologist. Hollenbeck PJ, Bamburg JR. Methods Cell Biol. 2003;71:1-16. Review.
Preservation of Cell Culture Stocks in Liquid Nitrogen Hay, R. J., 1978, pages 787-790. TCA Manual 4.
A Simple Method for Freezing and Thawing Cultured Cells Schroy, C. B., and P. Todd, 1976, pages 309-310. TCA Manual 2, Procedure
Number 76035.
Freezing, Storage, and Recovery of Cell Stocks Shannon, J. E. and M. L. Macy, 1973, pages 712-718. In Tissue Culture: Methods and Applications.
P. F. Kruse and M. K. Patterson Jr. Eds. (Academic Press, New York).
Simple Freezing Procedure for Storage in Serum-free Media of Cultured and Tumor Cells of Mouse Waymouth, C. and D. S. Varnum, 1976,
pages 311-313. TCA Manual 2, Procedure Number 76165.
The storage of cells from different tumor lines in a mechanical freezer at -80 degrees C. Comparison to cryopreservation in liquid nitrogen.
Ratajczak MZ, Kregenow DA, Kuczynski WI, Ratajczak J, Gewirtz AM., Mater Med Pol.1994 Apr-Jun;26(2):69-72.
view abstract
Cell culture contamination: an overview. Langdon SP, Methods Mol Med. 2004;88:309-17. Review.
view abstract
A list of related PubMed articles on Cell Culture Contaminations
Home Page
About Project
Project
Maps
Search
Page
Lectures
Lab01 Background
Lab02 Introduction to Tissue Culture
Medicine
Society & Health
Science
General
Help ??
Medicine Homepage
WebCT
NCBI Bookshelf
This Lab is an introduction to cell culture, also called tissue culture. As such, I expect at this stage only a broad understanding of concepts, that will be built upon in later Labs.
In Cell Biology, we need to be able to study individual cells, which can be difficult in the original animal or plant (in vivo). Therefore cell culture allows us to study (in vitro) individual, groups of, and whole tissues of cells.
From a beginnining early last century we have learnt the nutritional requirements of cells for in vitro survival and developed a large number of research techniques based upon our ability to isolate and grow cells.
Using nearly identical tissue culture techniques different research labs can be carrying out diverse research projects (basic research, clinical, cancer, viral, stem cell, neurobiology, immunology, etc.)
Please email Dr Mark Hill if you wish to make a comment about this current project.