Enzyme-Linked ImmunoSorbent Assay (ELISA) an important method for immunologically identifying and quantifying proteins.
96 well Microtitre plate
Immunobiology - The detection, measurement, and characterization of antibodies and their use as research and diagnostic tools.
| Figure A.6. The principle of the enzyme-linked immunosorbent assay (ELISA).Biochemistry - Proteins Can Be Detected and Quantitated by Using an Enzyme-Linked Immunosorbent Assay |
Indirect ELISA and Sandwich ELISAUniversity of Arizona - The ELISA Method
Cell Signaling Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit #7190 |
Bobrovnik SA. Determination of antibody affinity by ELISA. Theory. J Biochem Biophys Methods. 2003 Sep 30;57(3):213-36.
Jordan W. Dual-antibody sandwich enzyme-linked immunosorbent assay. Methods Mol Biol. 2005;295:207-14.
Millipore - Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA) | Enzyme Substrates for ELISA Testing
Biocompare - ELISA-Light Immunoassay System from Applied Biosystems | What To Look For In A Plate Reader
IDEXX Labs - ELISA Technology
Modern Biology Inc | Diagnostic Automation, Inc | Molecular Devices - Microplate Reader |

There is a large number of different ELISA applications used by different Labs. This page covers only those intended for use in our Lab.
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