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UNSW Cell Biology

Methods- Enzyme-linked immunosorbent assay (ELISA)

Introduction

Enzyme-Linked ImmunoSorbent Assay (ELISA) an important method for immunologically identifying and quantifying proteins.

Microtitre Plate

96 well Microtitre plate

Links

Immunobiology - The detection, measurement, and characterization of antibodies and their use as research and diagnostic tools.

| Figure A.6. The principle of the enzyme-linked immunosorbent assay (ELISA).

Biochemistry - Proteins Can Be Detected and Quantitated by Using an Enzyme-Linked Immunosorbent Assay |

Indirect ELISA and Sandwich ELISA

University of Arizona - The ELISA Method

Assays

Cell Signaling Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit #7190 |

References

Bobrovnik SA. Determination of antibody affinity by ELISA. Theory. J Biochem Biophys Methods. 2003 Sep 30;57(3):213-36.

Jordan W. Dual-antibody sandwich enzyme-linked immunosorbent assay. Methods Mol Biol. 2005;295:207-14.

Search PubMed

ELISA | enzyme-linked immunosorbent assay

Links

Millipore - Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA) | Enzyme Substrates for ELISA Testing

Biocompare - ELISA-Light Immunoassay System from Applied Biosystems | What To Look For In A Plate Reader

IDEXX Labs - ELISA Technology

Modern Biology Inc | Diagnostic Automation, Inc | Molecular Devices - Microplate Reader |

Comments

Dr Mark Hill

There is a large number of different ELISA applications used by different Labs. This page covers only those intended for use in our Lab.

Please email Dr Mark Hill if you wish to make a comment about this current project.